{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:33:56Z","timestamp":1790645636298,"version":"4.1.0"},"reference-count":109,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,2,15]],"date-time":"2021-02-15T00:00:00Z","timestamp":1613347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["1R01 AI125565"],"award-info":[{"award-number":["1R01 AI125565"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Differentiation from asexual blood stages to mature sexual gametocytes is required for the transmission of malaria parasites. Here, we report that the ApiAP2 transcription factor, PfAP2\u2010G2 (PF3D7_1408200) plays a critical role in the maturation of\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    gametocytes. PfAP2\u2010G2 binds to the promoters of a wide array of genes that are expressed at many stages of the parasite life cycle. Interestingly, we also find binding of PfAP2\u2010G2 within the gene body of almost 3,000 genes, which strongly correlates with the location of H3K36me3 and several other histone modifications as well as Heterochromatin Protein 1 (HP1), suggesting that occupancy of PfAP2\u2010G2 in gene bodies may serve as an alternative regulatory mechanism. Disruption of\n                    <jats:italic>pfap2\u2010g2<\/jats:italic>\n                    does not impact asexual development, but the majority of sexual parasites are unable to mature beyond stage III gametocytes. The absence of\n                    <jats:italic>pfap2\u2010g2<\/jats:italic>\n                    leads to overexpression of 28% of the genes bound by PfAP2\u2010G2 and none of the PfAP2\u2010G2 bound genes are downregulated, suggesting that it is a repressor. We also find that PfAP2\u2010G2 interacts with chromatin remodeling proteins, a microrchidia (MORC) protein, and another ApiAP2 protein (PF3D7_1139300). Overall our data demonstrate that PfAP2\u2010G2 establishes an essential gametocyte maturation program in association with other chromatin\u2010related proteins.\n                  <\/jats:p>","DOI":"10.1111\/mmi.14676","type":"journal-article","created":{"date-parts":[[2020,12,25]],"date-time":"2020-12-25T23:20:43Z","timestamp":1608938443000},"page":"1005-1024","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["The PfAP2\u2010G2 transcription factor is a critical regulator of gametocyte maturation"],"prefix":"10.1111","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8271-7771","authenticated-orcid":false,"given":"Suprita","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology The Pennsylvania State University University Park PA USA"},{"name":"Huck Center for Malaria Research The Pennsylvania State University University Park PA 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