{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:16:16Z","timestamp":1789179376974,"version":"build-2803163510"},"reference-count":73,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["V001094"],"award-info":[{"award-number":["V001094"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                    is an opportunistic mold responsible for severe life\u2010threatening fungal infections in immunocompromised patients. The cell wall, an essential structure composed of glucan, chitin, and galactomannan, is considered to be a target for the development of antifungal drugs. The nucleotide sugar donor GDP\u2010mannose (GDP\u2010Man) is required for the biosynthesis of galactomannan, glycosylphosphatidylinositol (GPI) anchors, glycolipid, and protein glycosylation. Starting from fructose\u20106\u2010phosphate, GDP\u2010Man is produced by the sequential action of the enzymes phosphomannose isomerase, phosphomannomutase (Pmm), and GDP\u2010mannose pyrophosphorylase. Here, using heterokaryon rescue and gene knockdown approaches we demonstrate that the phosphomannomutase encoding gene in\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    (\n                    <jats:italic>pmmA<\/jats:italic>\n                    ) is essential for survival. Reduced expression of\n                    <jats:italic>pmmA<\/jats:italic>\n                    is associated with significant morphological defects including retarded germination, growth, reduced conidiation, and abnormal polarity. Moreover, the knockdown strain exhibited an altered cell wall organization and sensitivity toward cell wall perturbing agents. By solving the first crystal structure of\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    phosphomannomutase (\n                    <jats:italic>Af<\/jats:italic>\n                    PmmA) we identified non\u2010conservative substitutions near the active site when compared to the human orthologues. Taken together, this work provides a genetic and structural foundation for the exploitation of\n                    <jats:italic>Af<\/jats:italic>\n                    PmmA as a potential antifungal target.\n                  <\/jats:p>","DOI":"10.1111\/mmi.14706","type":"journal-article","created":{"date-parts":[[2021,2,24]],"date-time":"2021-02-24T23:06:47Z","timestamp":1614208007000},"page":"245-259","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Genetic and structural validation of phosphomannomutase as a cell wall target in\n                    <i>Aspergillus fumigatus<\/i>"],"prefix":"10.1111","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0854-6123","authenticated-orcid":false,"given":"Yuanwei","family":"Zhang","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory for Microbes and Functional Genomics Jiangsu Engineering and Technology Research Centre for Microbiology, College of Life Sciences, Nanjing Normal University Nanjing China"},{"name":"School of Life Sciences University of Dundee Dundee UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxia","family":"Fang","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Dundee Dundee UK"},{"name":"National Engineering Research Center for Non\u2010Food Biorefinery Guangxi Academy of Sciences Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olawale G.","family":"Raimi","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Dundee Dundee UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah E. A.","family":"Lockhart","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Dundee Dundee UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew T.","family":"Ferenbach","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Dundee Dundee UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Lu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Microbes and Functional Genomics Jiangsu Engineering and Technology Research Centre for Microbiology, College of Life Sciences, Nanjing Normal University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1499-6908","authenticated-orcid":false,"given":"Daan M. F.","family":"van Aalten","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Dundee Dundee UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2004.07.008"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-92-2-263"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2014.00145"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001625"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/mmy.39.1.9.17"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof3040057"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20441"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3004404"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.14.32"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro732"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.23.14107"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.02357-05"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.5.1.207-211.2006"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2005.04.002"},{"key":"e_1_2_9_16_1","first-page":"U313","article-title":"Use of PYMOL as a communications tool for molecular science","volume":"228","author":"DeLano W.L.","year":"2004","journal-title":"Abstracts of Papers of the American Chemical Society"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002940050103"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa6097"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.398321"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2016.109"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20130053"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12290"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.029975-0"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.457"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.23.8168-8183.2001"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.FUNK-0035-2016"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2009.01.003"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003276"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02647-18"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M704991200"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030024"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.8b00223"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2008\/019240-0"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/654251"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1402850111"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01610-05"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2006.08.023"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)76520-X"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.49.070180.004305"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1183\/09059180.00001011"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-030117-020406"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780400029155"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-016-6045-4"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2015.07.012"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0491907"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA120.012985"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444996012255"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444913017046"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-35059-2"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.406"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3074"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(97)76066-X"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3390201"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00673-0"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2006.02967.x"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm500066b"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.397"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(73)90388-4"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-030117-020345"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1087-1845(03)00090-2"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi3005126"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2012.08633.x"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.06.021"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M601505200"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1055"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.02391-05"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(83)90191-9"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.112"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2006.05.002"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb400411x"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1097\/00125817-200111000-00003"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/fov072"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.3369"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.14706","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mmi.14706","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.14706","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T07:15:23Z","timestamp":1693293323000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.14706"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3]]},"references-count":73,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["10.1111\/mmi.14706"],"URL":"https:\/\/doi.org\/10.1111\/mmi.14706","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3]]},"assertion":[{"value":"2020-10-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}