{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:45:45Z","timestamp":1747187145892,"version":"3.40.5"},"reference-count":66,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,11,17]],"date-time":"2021-11-17T00:00:00Z","timestamp":1637107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002910","name":"F\u00e9d\u00e9ration Wallonie-Bruxelles","doi-asserted-by":"publisher","award":["17\/22\u2010087"],"award-info":[{"award-number":["17\/22\u2010087"]}],"id":[{"id":"10.13039\/501100002910","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cyclic\u2010di\u2010GMP plays crucial role in the cell cycle regulation of the <jats:italic>\u03b1\u2010Proteobacterium<\/jats:italic> <jats:italic>Caulobacter crescentus<\/jats:italic>. Here we investigated its role in the <jats:italic>\u03b1\u2010Proteobacterium<\/jats:italic> <jats:italic>Brucella abortus<\/jats:italic>, a zoonotic intracellular pathogen. Surprisingly, deletion of all predicted cyclic\u2010di\u2010GMP synthesizing or degrading enzymes did not drastically impair the growth of <jats:italic>B.\u00a0abortus<\/jats:italic>, nor its ability to grow inside cell lines. As other Rhizobiales, <jats:italic>B.\u00a0abortus<\/jats:italic> displays unipolar growth from the new cell pole generated by cell division. We found that the phosphodiesterase PdeA, the ortholog of the essential polar growth factor RgsP of the Rhizobiale <jats:italic>Sinorhizobium meliloti<\/jats:italic>, is required for rod shape integrity but is not essential for <jats:italic>B.\u00a0abortus<\/jats:italic> growth. Indeed, the radius of the pole is increased by 31\u00a0\u00b1\u00a01.7% in a \u0394<jats:italic>pdeA<\/jats:italic> mutant, generating a coccoid morphology. A mutation in the cyclic\u2010di\u2010GMP phosphodiesterase catalytic site of PdeA does not generate the coccoid morphology and the \u0394<jats:italic>pdeA<\/jats:italic> mutant kept the ability to recruit markers of new and old poles. However, the presence of PdeA is required in an intra\u2010nasal mouse model of infection. In conclusion, we propose that PdeA contributes to bacterial morphology and virulence in <jats:italic>B.\u00a0abortus<\/jats:italic>, but it is not crucial for polarity and asymmetric growth.<\/jats:p>","DOI":"10.1111\/mmi.14833","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T19:47:12Z","timestamp":1634759232000},"page":"1449-1463","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["PdeA is required for the rod shape morphology of <i>Brucella abortus<\/i>"],"prefix":"10.1111","volume":"116","author":[{"given":"Ang\u00e9line","family":"Reboul","sequence":"first","affiliation":[{"name":"URBM Narilis University of Namur  Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elodie","family":"Carlier","sequence":"additional","affiliation":[{"name":"URBM Narilis University of Namur  Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois\u2010Xavier","family":"Stubbe","sequence":"additional","affiliation":[{"name":"URPHYM\u2010GEMO University of Namur  Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emeline","family":"Barbieux","sequence":"additional","affiliation":[{"name":"URBM Narilis University of Namur  Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aurore","family":"Demars","sequence":"additional","affiliation":[{"name":"URBM Narilis University of Namur  Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phuong Thi Anh","family":"Ong","sequence":"additional","affiliation":[{"name":"URBM Narilis University of Namur  Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antoine","family":"Gerodez","sequence":"additional","affiliation":[{"name":"URBM Narilis University of Namur  Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Muraille","sequence":"additional","affiliation":[{"name":"URBM Narilis University of Namur  Namur Belgium"},{"name":"Laboratoire de Parasitologie Universit\u00e9 Libre de Bruxelles and ULB Center for Research in Immunology (U\u2010CRI)  Gosselies Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3845-3043","authenticated-orcid":false,"given":"Xavier","family":"De Bolle","sequence":"additional","affiliation":[{"name":"URBM Narilis University of Namur  Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,17]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_9_2_1","DOI":"10.1371\/journal.pgen.1003744"},{"doi-asserted-by":"publisher","key":"e_1_2_9_3_1","DOI":"10.1016\/j.molcel.2011.07.018"},{"doi-asserted-by":"publisher","key":"e_1_2_9_4_1","DOI":"10.1046\/j.1365-2958.2003.03401.x"},{"doi-asserted-by":"publisher","key":"e_1_2_9_5_1","DOI":"10.1086\/319608"},{"doi-asserted-by":"publisher","key":"e_1_2_9_6_1","DOI":"10.1073\/pnas.1414622111"},{"doi-asserted-by":"publisher","key":"e_1_2_9_7_1","DOI":"10.1371\/journal.pone.0005893"},{"doi-asserted-by":"publisher","key":"e_1_2_9_8_1","DOI":"10.1111\/j.1365-2958.2010.07470.x"},{"doi-asserted-by":"publisher","key":"e_1_2_9_9_1","DOI":"10.1073\/pnas.1114476109"},{"doi-asserted-by":"publisher","key":"e_1_2_9_10_1","DOI":"10.1128\/microbiolspec.BAI-0006-2019"},{"doi-asserted-by":"publisher","key":"e_1_2_9_11_1","DOI":"10.1007\/s10096-002-0848-4"},{"doi-asserted-by":"publisher","key":"e_1_2_9_12_1","DOI":"10.1016\/S0732-8893(01)00344-3"},{"doi-asserted-by":"publisher","key":"e_1_2_9_13_1","DOI":"10.1038\/ncomms5366"},{"doi-asserted-by":"publisher","key":"e_1_2_9_14_1","DOI":"10.1186\/1471-2180-11-257"},{"doi-asserted-by":"publisher","key":"e_1_2_9_15_1","DOI":"10.1038\/nmicrobiol.2016.77"},{"doi-asserted-by":"publisher","key":"e_1_2_9_16_1","DOI":"10.1101\/gad.502409"},{"doi-asserted-by":"publisher","key":"e_1_2_9_17_1","DOI":"10.1111\/j.1365-313X.2006.02842.x"},{"doi-asserted-by":"publisher","key":"e_1_2_9_18_1","DOI":"10.1073\/pnas.2010199117"},{"key":"e_1_2_9_19_1","first-page":"233","article-title":"Brucellosis in humans\u2013etiology, diagnostics, clinical forms","volume":"20","author":"Galinska E.M.","year":"2013","journal-title":"Annals of Agricultural and Environmental Medicine"},{"doi-asserted-by":"publisher","key":"e_1_2_9_20_1","DOI":"10.1186\/1471-2180-5-35"},{"doi-asserted-by":"publisher","key":"e_1_2_9_21_1","DOI":"10.1128\/JB.00126-16"},{"doi-asserted-by":"publisher","key":"e_1_2_9_22_1","DOI":"10.1002\/wrna.1454"},{"doi-asserted-by":"publisher","key":"e_1_2_9_23_1","DOI":"10.1038\/sj.emboj.7601577"},{"doi-asserted-by":"publisher","key":"e_1_2_9_24_1","DOI":"10.4049\/jimmunol.1502265"},{"doi-asserted-by":"publisher","key":"e_1_2_9_25_1","DOI":"10.1128\/jb.177.21.6223-6229.1995"},{"doi-asserted-by":"publisher","key":"e_1_2_9_26_1","DOI":"10.15252\/embj.201797825"},{"doi-asserted-by":"publisher","key":"e_1_2_9_27_1","DOI":"10.1007\/978-3-540-75418-3_11"},{"doi-asserted-by":"publisher","key":"e_1_2_9_28_1","DOI":"10.1128\/JB.00253-19"},{"doi-asserted-by":"publisher","key":"e_1_2_9_29_1","DOI":"10.1038\/nrmicro.2016.190"},{"doi-asserted-by":"publisher","key":"e_1_2_9_30_1","DOI":"10.1038\/s41467-020-14585-6"},{"doi-asserted-by":"publisher","key":"e_1_2_9_31_1","DOI":"10.1128\/IAI.00531-16"},{"doi-asserted-by":"publisher","key":"e_1_2_9_32_1","DOI":"10.1128\/mBio.00306-20"},{"doi-asserted-by":"publisher","key":"e_1_2_9_33_1","DOI":"10.1073\/pnas.0511090103"},{"doi-asserted-by":"publisher","key":"e_1_2_9_34_1","DOI":"10.1002\/anie.201206749"},{"doi-asserted-by":"publisher","key":"e_1_2_9_35_1","DOI":"10.1021\/acschembio.9b00664"},{"doi-asserted-by":"publisher","key":"e_1_2_9_36_1","DOI":"10.1038\/nature14473"},{"doi-asserted-by":"publisher","key":"e_1_2_9_37_1","DOI":"10.1371\/journal.ppat.1000804"},{"doi-asserted-by":"publisher","key":"e_1_2_9_38_1","DOI":"10.1128\/JB.00066-10"},{"doi-asserted-by":"publisher","key":"e_1_2_9_39_1","DOI":"10.1111\/j.1462-2920.2011.02595.x"},{"doi-asserted-by":"publisher","key":"e_1_2_9_40_1","DOI":"10.1056\/NEJMra050570"},{"doi-asserted-by":"publisher","key":"e_1_2_9_41_1","DOI":"10.1016\/j.cell.2008.02.045"},{"doi-asserted-by":"publisher","key":"e_1_2_9_42_1","DOI":"10.1101\/gad.289504"},{"doi-asserted-by":"publisher","key":"e_1_2_9_43_1","DOI":"10.1073\/pnas.192319099"},{"doi-asserted-by":"publisher","key":"e_1_2_9_44_1","DOI":"10.1128\/JB.00428-11"},{"doi-asserted-by":"publisher","key":"e_1_2_9_45_1","DOI":"10.1146\/annurev-biochem-062917-012921"},{"doi-asserted-by":"publisher","key":"e_1_2_9_46_1","DOI":"10.1128\/JB.00165-08"},{"doi-asserted-by":"publisher","key":"e_1_2_9_47_1","DOI":"10.1128\/IAI.00647-17"},{"doi-asserted-by":"publisher","key":"e_1_2_9_48_1","DOI":"10.1073\/pnas.0600345103"},{"doi-asserted-by":"publisher","key":"e_1_2_9_49_1","DOI":"10.1128\/JB.187.5.1792-1798.2005"},{"doi-asserted-by":"publisher","key":"e_1_2_9_50_1","DOI":"10.1128\/JB.00795-15"},{"doi-asserted-by":"publisher","key":"e_1_2_9_51_1","DOI":"10.1371\/journal.pgen.1007594"},{"doi-asserted-by":"publisher","key":"e_1_2_9_52_1","DOI":"10.1128\/JB.187.14.4774-4781.2005"},{"doi-asserted-by":"publisher","key":"e_1_2_9_53_1","DOI":"10.1038\/nmeth.2089"},{"doi-asserted-by":"publisher","key":"e_1_2_9_54_1","DOI":"10.1073\/pnas.95.11.5857"},{"doi-asserted-by":"publisher","key":"e_1_2_9_55_1","DOI":"10.1038\/nbt1183-784"},{"doi-asserted-by":"publisher","key":"e_1_2_9_56_1","DOI":"10.3201\/eid0904.020576"},{"doi-asserted-by":"publisher","key":"e_1_2_9_57_1","DOI":"10.1128\/JB.00119-09"},{"doi-asserted-by":"publisher","key":"e_1_2_9_58_1","DOI":"10.1128\/IAI.00312-18"},{"doi-asserted-by":"publisher","key":"e_1_2_9_59_1","DOI":"10.1093\/gbe\/evx137"},{"doi-asserted-by":"publisher","key":"e_1_2_9_60_1","DOI":"10.3390\/ijms21207749"},{"doi-asserted-by":"publisher","key":"e_1_2_9_61_1","DOI":"10.1099\/jmm.0.46116-0"},{"doi-asserted-by":"publisher","key":"e_1_2_9_62_1","DOI":"10.1371\/journal.pone.0005519"},{"doi-asserted-by":"publisher","key":"e_1_2_9_63_1","DOI":"10.15252\/embj.2018100323"},{"key":"e_1_2_9_64_1","first-page":"292","article-title":"Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridization","volume":"34","author":"Verger J.\u2010M.","year":"1985","journal-title":"International Journal of Systematic and Evolutionary Microbiology"},{"doi-asserted-by":"publisher","key":"e_1_2_9_65_1","DOI":"10.1128\/JB.01091-13"},{"doi-asserted-by":"publisher","key":"e_1_2_9_66_1","DOI":"10.1128\/JB.01767-08"},{"doi-asserted-by":"publisher","key":"e_1_2_9_67_1","DOI":"10.1128\/mBio.02346-21"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.14833","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mmi.14833","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.14833","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T02:38:42Z","timestamp":1693190322000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.14833"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,17]]},"references-count":66,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1111\/mmi.14833"],"URL":"https:\/\/doi.org\/10.1111\/mmi.14833","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"type":"print","value":"0950-382X"},{"type":"electronic","value":"1365-2958"}],"subject":[],"published":{"date-parts":[[2021,11,17]]},"assertion":[{"value":"2021-05-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}