{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T01:49:07Z","timestamp":1791856147595,"version":"4.3.4"},"reference-count":72,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T00:00:00Z","timestamp":1645401600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"publisher","award":["MOH\u2010000145"],"award-info":[{"award-number":["MOH\u2010000145"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The conserved Tol\u2010Pal trans\u2010envelope complex is important for outer membrane (OM) stability and cell division in Gram\u2010negative bacteria. It is proposed to mediate OM constriction during cell division via cell wall tethering. Yet, recent studies suggest the complex has additional roles in OM lipid homeostasis and septal wall separation. How Tol\u2010Pal facilitates all these processes is unclear. To gain insights into its function(s), we applied transposon\u2010insertion sequencing, and report here a detailed network of genetic interactions with the\n                    <jats:italic>tol\u2010pal<\/jats:italic>\n                    locus in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    . We found one positive and\u00a0&gt;\u00a020 negative strong interactions based on fitness. Disrupting osmoregulated\u2010periplasmic glucan biosynthesis restores fitness and OM barrier function, but not proper division, in\n                    <jats:italic>tol\u2010pal<\/jats:italic>\n                    mutants. In contrast, deleting genes involved in OM homeostasis and cell wall remodeling causes synthetic growth defects in strains lacking Tol\u2010Pal, especially exacerbating OM barrier and\/or division phenotypes. Notably, the \u0394\n                    <jats:italic>tolA<\/jats:italic>\n                    mutant having additional defects in OM protein assembly (\u0394\n                    <jats:italic>bamB<\/jats:italic>\n                    ) exhibited severe division phenotypes, even when single mutants divided normally; this highlights the possibility for OM phenotypes to indirectly impact cell division. Overall, our work underscores the intricate nature of Tol\u2010Pal function, and reinforces its key roles in cell wall\u2010OM tethering, cell wall remodeling, and in particular, OM homeostasis.\n                  <\/jats:p>","DOI":"10.1111\/mmi.14882","type":"journal-article","created":{"date-parts":[[2022,1,23]],"date-time":"2022-01-23T05:40:02Z","timestamp":1642916402000},"page":"921-936","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Genetic interaction mapping highlights key roles of the\n                    <scp>Tol\u2010Pal<\/scp>\n                    complex"],"prefix":"10.1111","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5055-4517","authenticated-orcid":false,"given":"Wee Boon","family":"Tan","sequence":"first","affiliation":[{"name":"Department of Chemistry National University of Singapore  Singapore Singapore"},{"name":"Singapore Center for Environmental Life Sciences Engineering National University of Singapore (SCELSE\u2010NUS)  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5466-7183","authenticated-orcid":false,"given":"Shu\u2010Sin","family":"Chng","sequence":"additional","affiliation":[{"name":"Department of Chemistry National University of Singapore  Singapore Singapore"},{"name":"Singapore Center for Environmental Life Sciences Engineering National University of Singapore (SCELSE\u2010NUS)  Singapore 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