{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:43:41Z","timestamp":1747187021312,"version":"3.40.5"},"reference-count":82,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,5,16]],"date-time":"2022-05-16T00:00:00Z","timestamp":1652659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI146909","AI152983","AI159061"],"award-info":[{"award-number":["AI146909","AI152983","AI159061"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM133617"],"award-info":[{"award-number":["R35GM133617"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>M23 family endopeptidases play important roles in cell division and separation in a wide variety of bacteria. Recent studies have suggested that these proteins also contribute to bacterial virulence. However, the biological function of M23 peptidases in pathogenic spirochetes remains unexplored. Here, we describe <jats:italic>Borrelia burgdorferi<\/jats:italic>, the bacterial pathogen causing Lyme disease, requires a putative M23 family homolog, BB0761, for spirochete morphology and cell division. Indeed, the inactivation of <jats:italic>bb0761<\/jats:italic> led to an aberrant filamentous phenotype as well as the impairment of <jats:italic>B. burgdorferi<\/jats:italic> growth in vitro. These phenotypes were complemented not only with <jats:italic>B. burgdorferi bb0761<\/jats:italic>, but also with the <jats:italic>mepM<\/jats:italic> gene from <jats:italic>E. coli<\/jats:italic>. Moreover, the <jats:italic>bb0761<\/jats:italic> mutant showed a complete loss of infectivity in a murine model of Lyme borreliosis. Resistance of the mutant to osmotic and oxidative stresses was markedly reduced. Our combined results indicate that BB0761 contributes to <jats:italic>B. burgdorferi<\/jats:italic> cell division and virulence.<\/jats:p>","DOI":"10.1111\/mmi.14916","type":"journal-article","created":{"date-parts":[[2022,5,5]],"date-time":"2022-05-05T10:44:22Z","timestamp":1651747462000},"page":"1405-1418","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>BB0761<\/scp>, a <scp>MepM<\/scp> homolog, contributes to <i>Borrelia burgdorferi<\/i> cell division and mammalian infectivity"],"prefix":"10.1111","volume":"117","author":[{"given":"Christina","family":"Thompson","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine University of South Florida  Tampa Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sierra","family":"George","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine University of South Florida  Tampa Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria L.","family":"White","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Microbiology and Molecular Biology University of South Florida  Tampa Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4430-261X","authenticated-orcid":false,"given":"Prahathees J.","family":"Eswara","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Microbiology and Molecular Biology University of South Florida  Tampa Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7427-1856","authenticated-orcid":false,"given":"Zhiming","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine University of South Florida  Tampa Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,16]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_9_2_1","DOI":"10.1128\/AAC.48.5.1681-1687.2004"},{"doi-asserted-by":"publisher","key":"e_1_2_9_3_1","DOI":"10.1093\/infdis\/162.1.133"},{"key":"e_1_2_9_4_1","first-page":"959","article-title":"Chronic Lyme borreliosis in the laboratory mouse","volume":"143","author":"Barthold S.W.","year":"1993","journal-title":"The American Journal of Pathology"},{"key":"e_1_2_9_5_1","first-page":"263","article-title":"Kinetics of Borrelia burgdorferi dissemination and evolution of disease after intradermal inoculation of mice","volume":"139","author":"Barthold S.W.","year":"1991","journal-title":"The American Journal of Pathology"},{"doi-asserted-by":"publisher","key":"e_1_2_9_6_1","DOI":"10.1128\/AEM.02454-06"},{"doi-asserted-by":"publisher","key":"e_1_2_9_7_1","DOI":"10.1128\/JB.01721-08"},{"doi-asserted-by":"publisher","key":"e_1_2_9_8_1","DOI":"10.3389\/fmed.2021.666554"},{"doi-asserted-by":"publisher","key":"e_1_2_9_9_1","DOI":"10.1111\/j.1365-2958.2010.07383.x"},{"doi-asserted-by":"publisher","key":"e_1_2_9_10_1","DOI":"10.1111\/j.1365-2958.2008.06204.x"},{"issue":"153","key":"e_1_2_9_11_1","first-page":"59905","article-title":"Live\u2010cell fluorescence microscopy to investigate subcellular protein localization and cell morphology changes in bacteria","author":"Brzozowski R.S.","year":"2019","journal-title":"Journal of Visualized Experiments"},{"doi-asserted-by":"publisher","key":"e_1_2_9_12_1","DOI":"10.1111\/j.1365-2958.2008.06211.x"},{"doi-asserted-by":"publisher","key":"e_1_2_9_13_1","DOI":"10.1126\/science.7043737"},{"doi-asserted-by":"publisher","key":"e_1_2_9_14_1","DOI":"10.1128\/JB.187.22.7845-7852.2005"},{"doi-asserted-by":"publisher","key":"e_1_2_9_15_1","DOI":"10.1128\/IAI.72.11.6433-6445.2004"},{"doi-asserted-by":"publisher","key":"e_1_2_9_16_1","DOI":"10.3389\/fmicb.2019.01923"},{"doi-asserted-by":"publisher","key":"e_1_2_9_17_1","DOI":"10.1128\/mBio.00404-16"},{"doi-asserted-by":"publisher","key":"e_1_2_9_18_1","DOI":"10.1128\/MMBR.00217-20"},{"doi-asserted-by":"publisher","key":"e_1_2_9_19_1","DOI":"10.1111\/mmi.12213"},{"doi-asserted-by":"publisher","key":"e_1_2_9_20_1","DOI":"10.1371\/journal.ppat.1000676"},{"doi-asserted-by":"publisher","key":"e_1_2_9_21_1","DOI":"10.1111\/mmi.13221"},{"doi-asserted-by":"publisher","key":"e_1_2_9_22_1","DOI":"10.1128\/mBio.01136-18"},{"doi-asserted-by":"publisher","key":"e_1_2_9_23_1","DOI":"10.1128\/IAI.00186-17"},{"doi-asserted-by":"publisher","key":"e_1_2_9_24_1","DOI":"10.4269\/ajtmh.1995.53.397"},{"doi-asserted-by":"publisher","key":"e_1_2_9_25_1","DOI":"10.1128\/JB.188.5.1969-1978.2006"},{"doi-asserted-by":"publisher","key":"e_1_2_9_26_1","DOI":"10.1128\/mBio.02575-14"},{"doi-asserted-by":"publisher","key":"e_1_2_9_27_1","DOI":"10.3389\/fmicb.2021.729307"},{"doi-asserted-by":"publisher","key":"e_1_2_9_28_1","DOI":"10.1038\/37551"},{"doi-asserted-by":"publisher","key":"e_1_2_9_29_1","DOI":"10.1111\/mmi.12390"},{"doi-asserted-by":"publisher","key":"e_1_2_9_30_1","DOI":"10.1128\/JB.01828-06"},{"doi-asserted-by":"publisher","key":"e_1_2_9_31_1","DOI":"10.1128\/mBio.01204-17"},{"doi-asserted-by":"publisher","key":"e_1_2_9_32_1","DOI":"10.1111\/pim.12816"},{"doi-asserted-by":"publisher","key":"e_1_2_9_33_1","DOI":"10.1073\/pnas.231442498"},{"doi-asserted-by":"publisher","key":"e_1_2_9_34_1","DOI":"10.1111\/j.1365-2958.2007.05746.x"},{"doi-asserted-by":"publisher","key":"e_1_2_9_35_1","DOI":"10.1038\/s41579-020-0400-5"},{"doi-asserted-by":"publisher","key":"e_1_2_9_36_1","DOI":"10.1128\/IAI.69.1.446-455.2001"},{"doi-asserted-by":"publisher","key":"e_1_2_9_37_1","DOI":"10.1128\/JB.00571-10"},{"key":"e_1_2_9_38_1","first-page":"e18703","article-title":"Post\u2010treatment lyme disease syndrome: need for diagnosis and treatment","volume":"13","author":"Maksimyan S.","year":"2021","journal-title":"Cureus"},{"doi-asserted-by":"publisher","key":"e_1_2_9_39_1","DOI":"10.1111\/mmi.14504"},{"doi-asserted-by":"publisher","key":"e_1_2_9_40_1","DOI":"10.1111\/mmi.14460"},{"doi-asserted-by":"publisher","key":"e_1_2_9_41_1","DOI":"10.1038\/ncomms5269"},{"doi-asserted-by":"publisher","key":"e_1_2_9_42_1","DOI":"10.1016\/j.mib.2015.09.006"},{"doi-asserted-by":"publisher","key":"e_1_2_9_43_1","DOI":"10.1099\/mic.0.2008\/019992-0"},{"doi-asserted-by":"publisher","key":"e_1_2_9_44_1","DOI":"10.1073\/pnas.0812999106"},{"doi-asserted-by":"publisher","key":"e_1_2_9_45_1","DOI":"10.1186\/1471-2180-12-44"},{"doi-asserted-by":"publisher","key":"e_1_2_9_46_1","DOI":"10.1086\/523764"},{"key":"e_1_2_9_47_1","first-page":"113","article-title":"Interactions between ticks and lyme disease spirochetes","volume":"42","author":"Pal U.","year":"2021","journal-title":"Current Issues in Molecular Biology"},{"doi-asserted-by":"publisher","key":"e_1_2_9_48_1","DOI":"10.1016\/j.cell.2004.10.027"},{"doi-asserted-by":"publisher","key":"e_1_2_9_49_1","DOI":"10.1111\/mmi.12304"},{"doi-asserted-by":"publisher","key":"e_1_2_9_50_1","DOI":"10.1128\/JCM.31.5.1251-1255.1993"},{"doi-asserted-by":"publisher","key":"e_1_2_9_51_1","DOI":"10.1073\/pnas.97.25.13865"},{"doi-asserted-by":"publisher","key":"e_1_2_9_52_1","DOI":"10.1038\/nrmicro2714"},{"doi-asserted-by":"publisher","key":"e_1_2_9_53_1","DOI":"10.1128\/jb.179.11.3625-3631.1997"},{"doi-asserted-by":"publisher","key":"e_1_2_9_54_1","DOI":"10.1093\/nar\/gkx1134"},{"doi-asserted-by":"publisher","key":"e_1_2_9_55_1","DOI":"10.1111\/mmi.12377"},{"doi-asserted-by":"publisher","key":"e_1_2_9_56_1","DOI":"10.1146\/annurev.micro.112408.134040"},{"key":"e_1_2_9_57_1","first-page":"223","article-title":"Gene regulation and transcriptomics","volume":"42","author":"Samuels D.S.","year":"2021","journal-title":"Current Issues in Molecular Biology"},{"key":"e_1_2_9_58_1","first-page":"167","article-title":"Ticks and Borrelia: model systems for investigating pathogen\u2010arthropod interactions","volume":"5","author":"Schwan T.G.","year":"1996","journal-title":"Infectious Agents and Disease"},{"doi-asserted-by":"publisher","key":"e_1_2_9_59_1","DOI":"10.1111\/j.1365-2958.2004.04352.x"},{"doi-asserted-by":"publisher","key":"e_1_2_9_60_1","DOI":"10.1111\/j.1574-6968.2010.02087.x"},{"doi-asserted-by":"publisher","key":"e_1_2_9_61_1","DOI":"10.1111\/mmi.12058"},{"doi-asserted-by":"publisher","key":"e_1_2_9_62_1","DOI":"10.1016\/j.jmb.2009.12.021"},{"doi-asserted-by":"publisher","key":"e_1_2_9_63_1","DOI":"10.1080\/21548331.1993.11442776"},{"doi-asserted-by":"publisher","key":"e_1_2_9_64_1","DOI":"10.1172\/JCI21681"},{"doi-asserted-by":"publisher","key":"e_1_2_9_65_1","DOI":"10.1016\/j.plasmid.2004.10.006"},{"doi-asserted-by":"publisher","key":"e_1_2_9_66_1","DOI":"10.1074\/jbc.M111.338830"},{"doi-asserted-by":"publisher","key":"e_1_2_9_67_1","DOI":"10.1128\/mBio.00399-13"},{"doi-asserted-by":"publisher","key":"e_1_2_9_68_1","DOI":"10.1016\/j.jgar.2020.12.025"},{"doi-asserted-by":"publisher","key":"e_1_2_9_69_1","DOI":"10.1128\/IAI.00951-19"},{"doi-asserted-by":"publisher","key":"e_1_2_9_70_1","DOI":"10.1371\/journal.pone.0007023"},{"doi-asserted-by":"publisher","key":"e_1_2_9_71_1","DOI":"10.1371\/journal.pone.0084625"},{"doi-asserted-by":"publisher","key":"e_1_2_9_72_1","DOI":"10.1128\/JB.00505-09"},{"doi-asserted-by":"publisher","key":"e_1_2_9_73_1","DOI":"10.1038\/emboj.2010.36"},{"doi-asserted-by":"publisher","key":"e_1_2_9_74_1","DOI":"10.3389\/fmicb.2019.00331"},{"doi-asserted-by":"publisher","key":"e_1_2_9_75_1","DOI":"10.1080\/19490976.2020.1799654"},{"doi-asserted-by":"publisher","key":"e_1_2_9_76_1","DOI":"10.1007\/s12016-021-08906-w"},{"doi-asserted-by":"publisher","key":"e_1_2_9_77_1","DOI":"10.3389\/fcimb.2017.00131"},{"doi-asserted-by":"publisher","key":"e_1_2_9_78_1","DOI":"10.1073\/pnas.1834315100"},{"doi-asserted-by":"publisher","key":"e_1_2_9_79_1","DOI":"10.1111\/mpp.12653"},{"doi-asserted-by":"publisher","key":"e_1_2_9_80_1","DOI":"10.1111\/j.1574-6968.2008.01416.x"},{"doi-asserted-by":"publisher","key":"e_1_2_9_81_1","DOI":"10.1128\/IAI.00360-16"},{"doi-asserted-by":"publisher","key":"e_1_2_9_82_1","DOI":"10.1007\/s11427-015-4996-z"},{"doi-asserted-by":"publisher","key":"e_1_2_9_83_1","DOI":"10.1111\/mmi.14243"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.14916","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mmi.14916","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.14916","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T07:49:30Z","timestamp":1692949770000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.14916"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,16]]},"references-count":82,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,6]]}},"alternative-id":["10.1111\/mmi.14916"],"URL":"https:\/\/doi.org\/10.1111\/mmi.14916","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"type":"print","value":"0950-382X"},{"type":"electronic","value":"1365-2958"}],"subject":[],"published":{"date-parts":[[2022,5,16]]},"assertion":[{"value":"2022-01-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}