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Our 1.4\u00a0\u00c5 crystal structure of the membrane\u2010proximal part of the BpaC head domain shows that the domain is exclusively made of left\u2010handed parallel \u03b2\u2010roll repeats. This, the largest such structure solved, has two unique features. First, the core, rather than being composed of the canonical hydrophobic Ile and Val, is made up primarily of the hydrophilic Thr and Asn, with two different solvent channels. Second, comparing BpaC to all other left\u2010handed parallel \u03b2\u2010roll structures showed that the position of the head domain in the protein correlates with the number and type of charged residues. In BpaC, only negatively charged residues face the solvent\u2014in stark contrast to the primarily positive surface charge of the left\u2010handed parallel \u03b2\u2010roll \u201ctype\u201d protein, YadA. We propose extending the definitions of these head domains to include the BpaC\u2010like head domain as a separate subtype, based on its unusual sequence, position, and charge. We speculate that the function of left\u2010handed parallel \u03b2\u2010roll structures may differ depending on their position in the structure.<\/jats:p>","DOI":"10.1111\/mmi.14953","type":"journal-article","created":{"date-parts":[[2022,6,15]],"date-time":"2022-06-15T12:40:27Z","timestamp":1655296827000},"page":"77-91","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The C\u2010terminal head domain of <i>Burkholderia pseudomallei<\/i><scp>BpaC<\/scp> has a striking hydrophilic core with an extensive solvent network"],"prefix":"10.1111","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9575-8153","authenticated-orcid":false,"given":"Andreas R.","family":"Kiessling","sequence":"first","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology School of Biomedical Sciences, University of Leeds  Leeds UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2812-1651","authenticated-orcid":false,"given":"Sarah A.","family":"Harris","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology, School of Physics and Astronomy University of Leeds  Leeds UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2937-2994","authenticated-orcid":false,"given":"Kathleen M.","family":"Weimer","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology School of Biomedical Sciences, University of Leeds  Leeds UK"},{"name":"IGBMC, School of Life and Health Sciences University of Strasbourg  Strasbourg France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0253-911X","authenticated-orcid":false,"given":"Geoffrey","family":"Wells","sequence":"additional","affiliation":[{"name":"UCL School of Pharmacy University College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8032-9700","authenticated-orcid":false,"given":"Adrian","family":"Goldman","sequence":"additional","affiliation":[{"name":"Astbury Centre for Structural Molecular Biology School of Biomedical Sciences, University of Leeds  Leeds UK"},{"name":"MIBS, Faculty of Biological and Environmental Sciences University of Helsinki  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2011.07.005"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1106341108"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022\u20102836(05)80360\u20102"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102180\u201010\u2010250"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2014.12.010"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx343"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa977"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz297"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1004"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01218\u201010"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00526\u201013"},{"key":"e_1_2_10_14_1","volume-title":"Amber 2021","author":"Case D.A.","year":"2021"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e32833fb88c"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2008.101"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444906022116"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444911039655"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.849004"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/3039106"},{"key":"e_1_2_10_21_1","doi-asserted-by":"crossref","unstructured":"Edwards T. 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