{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:36:04Z","timestamp":1790980564988,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T00:00:00Z","timestamp":1657065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002661","name":"Fonds De La Recherche Scientifique - FNRS","doi-asserted-by":"publisher","award":["T.0058.20"],"award-info":[{"award-number":["T.0058.20"]}],"id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002661","name":"Fonds De La Recherche Scientifique - FNRS","doi-asserted-by":"publisher","award":["T.0060.15"],"award-info":[{"award-number":["T.0060.15"]}],"id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The pathogenic bacterium\n                    <jats:italic>Brucella abortus<\/jats:italic>\n                    invades and multiplies inside host cells. To grow inside host cells,\n                    <jats:italic>B. abortus<\/jats:italic>\n                    requires a functional histidine biosynthesis pathway. Here, we show that a\n                    <jats:italic>B. abortus<\/jats:italic>\n                    histidine auxotroph mutant also displays an unexpected chaining phenotype. The intensity of this phenotype varies according to the culture medium and is exacerbated inside host cells. Chains of bacteria consist of contiguous peptidoglycan, and likely result from the defective cleavage of peptidoglycan at septa. Genetic suppression of the chaining phenotype unearthed two essential genes with a role in\n                    <jats:italic>B. abortus<\/jats:italic>\n                    cell division:\n                    <jats:italic>dipM<\/jats:italic>\n                    and\n                    <jats:italic>cdlP<\/jats:italic>\n                    . Loss of function of\n                    <jats:italic>dipM<\/jats:italic>\n                    and\n                    <jats:italic>cdlP<\/jats:italic>\n                    generates swelling at the division site. While DipM is strictly localized at the division site, CdlP is localized at the growth pole and the division site. Altogether, the unexpected chaining phenotype of a\n                    <jats:italic>hisB<\/jats:italic>\n                    mutant allowed the discovery of new crucial actors in cell division in\n                    <jats:italic>B. abortus<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/mmi.14956","type":"journal-article","created":{"date-parts":[[2022,6,24]],"date-time":"2022-06-24T05:33:38Z","timestamp":1656048818000},"page":"145-154","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Histidine auxotroph mutant is defective for cell separation and allows the identification of crucial factors for cell division in\n                    <i>Brucella abortus<\/i>"],"prefix":"10.1111","volume":"118","author":[{"given":"Agn\u00e8s","family":"Roba","sequence":"first","affiliation":[{"name":"Research Unit in Biology of Microorganisms, Narilis University of Namur  Namur 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