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To cross host barriers and establish infection, <jats:italic>Lm<\/jats:italic> is dependent upon the regulated secretion and activity of many proteins including PrsA2, a peptidyl\u2010prolyl <jats:italic>cis\u2010trans<\/jats:italic> isomerase with foldase activity. PrsA2 contributes to the stability and activity of a number of secreted virulence factors that are required for <jats:italic>Lm<\/jats:italic> invasion, replication, and cell\u2010to\u2010cell spread within the infected host. In contrast, a second related secretion chaperone, PrsA1, has thus far no identified contributions to <jats:italic>Lm<\/jats:italic> pathogenesis. Here we describe the characterization of a two\u2010component signal transduction system PieRS that regulates the expression of a regulon that includes the secretion chaperones PrsA1 and PrsA2. PieRS regulated gene products are required for bacterial resistance to ethanol exposure and are important for bacterial survival during transit through the gastrointestinal tract. PrsA1 was also found to make a unique contribution to <jats:italic>Lm<\/jats:italic> survival in the GI tract, revealing for the first time a non\u2010overlapping requirement for both secretion chaperones PrsA1 and PrsA2 during the process of intra\u2010gastric infection.<\/jats:p>","DOI":"10.1111\/mmi.14967","type":"journal-article","created":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T05:44:45Z","timestamp":1659332685000},"page":"278-293","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["<i>Listeria monocytogenes<\/i> two component system PieRS regulates secretion chaperones PrsA1 and PrsA2 and enhances bacterial translocation across the intestine"],"prefix":"10.1111","volume":"118","author":[{"given":"Laty A.","family":"Cahoon","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology University of Illinois at Chicago  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiomarie","family":"Alejandro\u2010Navarreto","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Illinois at Chicago  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7500-9670","authenticated-orcid":false,"given":"Avinash N.","family":"Gururaja","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Illinois at Chicago  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sam H.","family":"Light","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Chicago  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Alonzo","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Loyola University  Chicago 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