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Riboflavin salvage has been shown to be important for <jats:italic>B. burgdorferi<\/jats:italic> infection of mice, yet the contributions of riboflavin to <jats:italic>B. burgdorferi<\/jats:italic> metabolism and survival in the tick remain unknown. Using a targeted mass spectrometry approach, we confirmed the importance of <jats:italic>bb0318<\/jats:italic>, the putative ATPase component of an ABC\u2010type riboflavin transporter, for riboflavin salvage and the production of FMN and FAD. This analysis further revealed that \u0394<jats:italic>bb0318 B. burgdorferi<\/jats:italic> displayed increased levels of glycerol 3\u2010phosphate compared to the wild\u2010type. The glycerol 3\u2010phosphate dehydrogenase activity of GlpD was found to be FAD\u2010dependent and the transcription and translation of <jats:italic>glpD<\/jats:italic> were significantly decreased in \u0394<jats:italic>bb0318 B. burgdorferi<\/jats:italic>. Finally, gene <jats:italic>bb0318<\/jats:italic> was found to be important for maximal spirochete burden in unfed larvae and essential for survival in feeding ticks. Together, these data demonstrate the importance of riboflavin salvage for <jats:italic>B. burgdorferi<\/jats:italic> carbon metabolism and survival in ticks.<\/jats:p>","DOI":"10.1111\/mmi.14977","type":"journal-article","created":{"date-parts":[[2022,8,27]],"date-time":"2022-08-27T10:05:59Z","timestamp":1661594759000},"page":"443-456","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Riboflavin salvage by <i>Borrelia burgdorferi<\/i> supports carbon metabolism and is essential for survival in the tick vector"],"prefix":"10.1111","volume":"118","author":[{"given":"Darlene","family":"Ramos","sequence":"first","affiliation":[{"name":"Division of Immunity and Pathogenesis, Burnett School of Biomedical Sciences, College of Medicine University of Central Florida  Orlando Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda 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