{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T18:35:02Z","timestamp":1772390102688,"version":"3.50.1"},"reference-count":65,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T00:00:00Z","timestamp":1673827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201010\u2010EQPX\u201004\u201001"],"award-info":[{"award-number":["ANR\u201010\u2010EQPX\u201004\u201001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201016\u2010CE35\u20100009"],"award-info":[{"award-number":["ANR\u201016\u2010CE35\u20100009"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201018\u2010JAM2\u20100002"],"award-info":[{"award-number":["ANR\u201018\u2010JAM2\u20100002"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["CycloNHit 608407"],"award-info":[{"award-number":["CycloNHit 608407"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["ERC\u2010STG INTRACELLTB 260901"],"award-info":[{"award-number":["ERC\u2010STG INTRACELLTB 260901"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["MM4TB 260872"],"award-info":[{"award-number":["MM4TB 260872"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002924","name":"Federaci\u00f3n Espa\u00f1ola de Enfermedades Raras","doi-asserted-by":"publisher","award":["12001407 (D\u2010AL) Equipex Imaginex BioMed"],"award-info":[{"award-number":["12001407 (D\u2010AL) Equipex Imaginex BioMed"]}],"id":[{"id":"10.13039\/501100002924","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"publisher","award":["J558"],"award-info":[{"award-number":["J558"]}],"id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"publisher","award":["PTR430"],"award-info":[{"award-number":["PTR430"]}],"id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"publisher","award":["PTR441"],"award-info":[{"award-number":["PTR441"]}],"id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["Ci\u00eancia Sem Fronteiras"],"award-info":[{"award-number":["Ci\u00eancia Sem Fronteiras"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tuberculosis remains a global health threat with high morbidity. Dendritic cells (DCs) participate in the acute and chronic inflammatory responses to <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (Mtb) by directing the adaptive immune response and are present in lung granulomas. In macrophages, the interaction of lipid droplets (LDs) with mycobacteria\u2010containing phagosomes is central to host\u2010pathogen interactions. However, the data available for DCs are still a matter of debate. Here, we reported that bone marrow\u2010derived DCs (BMDCs) were susceptible to Mtb infection and replication at similar rate to macrophages. Unlike macrophages, the analysis of gene expression showed that Mtb infection induced a delayed increase in lipid droplet\u2010related genes and proinflammatory response. Hence, LD accumulation has been observed by high\u2010content imaging in late periods. Infection of BMDCs with killed H37Rv demonstrated that LD accumulation depends on Mtb viability. Moreover, infection with the attenuated strains H37Ra and <jats:italic>Mycobacterium bovis<\/jats:italic>\u2010BCG induced only an early transient increase in LDs, whereas virulent Mtb also induced delayed LD accumulation. In addition, infection with the BCG strain with the reintroduced virulence RD1 locus induced higher LD accumulation and bacterial replication when compared to parental BCG. Collectively, our data suggest that delayed LD accumulation in DCs is dependent on mycobacterial viability and virulence.<\/jats:p>","DOI":"10.1111\/mmi.15023","type":"journal-article","created":{"date-parts":[[2022,12,29]],"date-time":"2022-12-29T09:26:13Z","timestamp":1672305973000},"page":"224-236","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<i>Mycobacterium tuberculosis<\/i> induces delayed lipid droplet accumulation in dendritic cells depending on bacterial viability and virulence"],"prefix":"10.1111","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2300-5384","authenticated-orcid":false,"given":"Maria Fernanda de Souza","family":"Costa","sequence":"first","affiliation":[{"name":"Instituto de Biologia, Departamento de Imunobiologia Universidade Federal Fluminense  Niteroi Brazil"},{"name":"Center for Technological Development in Health Funda\u00e7\u00e3o Oswaldo Cruz  Rio de Janeiro Brazil"},{"name":"Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille U1019 \u2013 UMR 9017 \u2010 CIIL \u2010 Center for Infection and Immunity of Lille  Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1917-2876","authenticated-orcid":false,"given":"Filipe","family":"Pereira\u2010Dutra","sequence":"additional","affiliation":[{"name":"Immunopharmacology Laboratory Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Deboosere","sequence":"additional","affiliation":[{"name":"Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille U1019 \u2013 UMR 9017 \u2010 CIIL \u2010 Center for Infection and Immunity of Lille  Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel","family":"Jouny","sequence":"additional","affiliation":[{"name":"Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille U1019 \u2013 UMR 9017 \u2010 CIIL \u2010 Center for Infection and Immunity of Lille  Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ok\u2010Ryul","family":"Song","sequence":"additional","affiliation":[{"name":"Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille U1019 \u2013 UMR 9017 \u2010 CIIL \u2010 Center for Infection and Immunity of Lille  Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guilherme","family":"Iack","sequence":"additional","affiliation":[{"name":"Instituto de Biologia, Departamento de Imunobiologia Universidade Federal Fluminense  Niteroi Brazil"},{"name":"Immunopharmacology Laboratory Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreia Lamoglia","family":"Souza","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Laboratory of Immunology and Immunogenetics in Infectious Diseases at Evandro Chagas National Institute of Infectious Diseases  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric Henrique","family":"Roma","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Laboratory of Immunology and Immunogenetics in Infectious Diseases at Evandro Chagas National Institute of Infectious Diseases  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5235-7069","authenticated-orcid":false,"given":"Vincent","family":"Delorme","sequence":"additional","affiliation":[{"name":"Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille U1019 \u2013 UMR 9017 \u2010 CIIL \u2010 Center for Infection and Immunity of Lille  Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia T.","family":"Bozza","sequence":"additional","affiliation":[{"name":"Immunopharmacology Laboratory Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priscille","family":"Brodin","sequence":"additional","affiliation":[{"name":"Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille U1019 \u2013 UMR 9017 \u2010 CIIL \u2010 Center for Infection and Immunity of Lille  Lille France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,1,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0900365"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006095"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12437"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1139\/y59-099"},{"key":"e_1_2_10_6_1","article-title":"Mammalian lipid droplets are innate immune hubs integrating cell metabolism and host defense","volume":"1979","author":"Bosch M.","year":"2020","journal-title":"Science"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.06.022"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI141833"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00488-09"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.7.4203"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01196-13"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000645"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.1.257"},{"key":"e_1_2_10_14_1","first-page":"7","article-title":"Mycobacterium tuberculosis uses host triacylglycerol to accumulate lipid droplets and acquires a dormancy\u2010like phenotype in lipid\u2010loaded macrophages","author":"Daniel J.","year":"2011","journal-title":"PLoS Pathogens"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-022-00731-y"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.5.3087"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.722735"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1460-0_29"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep17078"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.2.978"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep09613"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2172"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)41065-3"},{"key":"e_1_2_10_24_1","first-page":"307631","article-title":"Immunopathology of postprimary tuberculosis: increased T\u2010regulatory cells and DEC\u2010205\u2010positive foamy macrophages in cavitary lesions","volume":"2011","author":"Hunter R.L.","year":"2011","journal-title":"Clinical & Developmental Immunology"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00088"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053657"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2015.68"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12951"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.8771"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006874"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12952"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002276"},{"key":"e_1_2_10_33_1","article-title":"IRG1 controls host responses to restrict Mycobacterium tuberculosis infection","author":"Machelart A.","year":"2022","journal-title":"bioRxiv"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1103038"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.6.3570"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101344"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100064"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1569-2"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/402827"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.1.104-109.2003"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1252"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01512-13"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2013.09.002"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20151570"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.6.3873"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0711159105"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.4MR0119-035R"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000204"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03237.x"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2010.04168.x"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.4MR0520-112R"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2020.158703"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M054874"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2016.00122"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI45113"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2015.02.019"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2012.09.012"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.23129"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12217"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.4.1939"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.5.3268"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI77014"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1006\/clim.2002.5287"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.4.2509"},{"key":"e_1_2_10_65_1","volume-title":"Global tuberculosis report 2022","author":"World Health Organization","year":"2022"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.058198-0"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.15023","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mmi.15023","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.15023","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T03:13:43Z","timestamp":1692587623000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.15023"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,16]]},"references-count":65,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["10.1111\/mmi.15023"],"URL":"https:\/\/doi.org\/10.1111\/mmi.15023","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,16]]},"assertion":[{"value":"2021-11-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}