{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T04:18:56Z","timestamp":1785817136602,"version":"3.56.0"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,24]],"date-time":"2023-01-24T00:00:00Z","timestamp":1674518400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 GM122947"],"award-info":[{"award-number":["R01 GM122947"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In <jats:italic>Bradyrhizobium japonicum<\/jats:italic>, iron uptake from ferric siderophores involves selective outer membrane proteins and non\u2010selective periplasmic and cytoplasmic membrane components that accommodate numerous structurally diverse siderophores. Free iron traverses the cytoplasmic membrane through the ferrous (Fe<jats:sup>2+<\/jats:sup>) transporter system FeoAB, but the other non\u2010selective components have not been described. Here, we identify <jats:italic>fsrB<\/jats:italic> as an iron\u2010regulated gene required for growth on iron chelates of catecholate\u2010 and hydroxymate\u2010type siderophores, but not on inorganic iron. Utilization of the non\u2010physiological iron chelator EDDHA as an iron source was also dependent on <jats:italic>fsrB<\/jats:italic>. Uptake activities of <jats:sup>55<\/jats:sup>Fe<jats:sup>3+<\/jats:sup> bound to ferrioxamine B, ferrichrome or enterobactin were severely diminished in the <jats:italic>fsrB<\/jats:italic> mutant compared with the wild type. Growth of the <jats:italic>fsrB<\/jats:italic> or <jats:italic>feoB<\/jats:italic> strains on ferrichrome were rescued with plasmid\u2010borne <jats:italic>E. coli fhuCDB<\/jats:italic> ferrichrome transport genes, suggesting that FsrB activity occurs in the periplasm rather than the cytoplasm. Whole cells of an <jats:italic>fsrB<\/jats:italic> mutant are defective in ferric reductase activity. Both whole cells and spheroplasts catalyzed the demetallation of ferric siderophores that were defective in an <jats:italic>fsrB<\/jats:italic> mutant. Collectively, the data support a model whereby FsrB is required for reduction of iron and its dissociation from the siderophore in the periplasm, followed by transport of the ferrous ion into the cytoplasm by FeoAB.<\/jats:p>","DOI":"10.1111\/mmi.15028","type":"journal-article","created":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T15:06:29Z","timestamp":1673967989000},"page":"340-349","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The <i>Bradyrhizobium japonicum fsrB<\/i> gene is essential for utilization of structurally diverse ferric siderophores to fulfill its nutritional iron requirement"],"prefix":"10.1111","volume":"119","author":[{"given":"Alasteir","family":"Ong","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences The University at Buffalo  Buffalo New York 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