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One conserved feature of these invasive forms is the micronemes, apically oriented secretory organelles involved in egress, motility, adhesion, and invasion. Here we investigate the role of GPI\u2010anchored micronemal antigen (GAMA), which shows a micronemal localization in all zoite forms of the rodent\u2010infecting species <jats:italic>Plasmodium berghei.<\/jats:italic> \u2206GAMA parasites are severely defective for invasion of the mosquito midgut. Once formed, oocysts develop normally, however, sporozoites are unable to egress and exhibit defective motility. Epitope\u2010tagging of GAMA revealed tight temporal expression late during sporogony and showed that GAMA is shed during sporozoite gliding motility in a similar manner to circumsporozoite protein. Complementation of <jats:italic>P. berghei<\/jats:italic> knockout parasites with full\u2010length <jats:italic>P. falciparum<\/jats:italic> GAMA partially restored infectivity to mosquitoes, indicating conservation of function across <jats:italic>Plasmodium<\/jats:italic> species. A suite of parasites with GAMA expressed under the promoters of <jats:italic>CTRP<\/jats:italic>, <jats:italic>CAP380<\/jats:italic>, and <jats:italic>TRAP<\/jats:italic>, further confirmed the involvement of GAMA in midgut infection, motility, and vertebrate infection. These data show GAMA's involvement in sporozoite motility, egress, and invasion, implicating GAMA as a regulator of microneme function.<\/jats:p>","DOI":"10.1111\/mmi.15078","type":"journal-article","created":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T17:05:53Z","timestamp":1686762353000},"page":"394-412","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>Plasmodium<\/i><scp>GPI\u2010anchored<\/scp> micronemal antigen is essential for parasite transmission through the mosquito host"],"prefix":"10.1111","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8138-9999","authenticated-orcid":false,"given":"Charlie","family":"Jennison","sequence":"first","affiliation":[{"name":"Center for Global Infectious Disease Research Seattle Children's Research Institute  Washington Seattle USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janna M.","family":"Armstrong","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research Seattle Children's Research Institute  Washington Seattle USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorender A.","family":"Dankwa","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research Seattle Children's Research Institute  Washington Seattle USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina","family":"Hertoghs","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research Seattle Children's Research Institute  Washington Seattle USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sudhir","family":"Kumar","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research Seattle Children's Research Institute  Washington Seattle USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Biley A.","family":"Abatiyow","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research Seattle Children's Research Institute  Washington Seattle USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myo","family":"Naung","sequence":"additional","affiliation":[{"name":"Population Health and Immunity Division Walter and Eliza Hall Institute of Medical Research  Victoria Parkville Australia"},{"name":"Department of Medical Biology University of Melbourne  Victoria Carlton Australia"},{"name":"Department of Global Health University of Washington  Washington Seattle USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nana K.","family":"Minkah","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research Seattle Children's Research Institute  Washington Seattle USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristian E.","family":"Swearingen","sequence":"additional","affiliation":[{"name":"Institute of Mental and Physical Health and Clinical Translation (IMPACT) School of Medicine, Deakin University  Victoria Geelong Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Moritz","sequence":"additional","affiliation":[{"name":"Institute of Mental and Physical Health and Clinical Translation (IMPACT) School of Medicine, Deakin University  Victoria Geelong Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alyssa E.","family":"Barry","sequence":"additional","affiliation":[{"name":"Department of Global Health University of Washington  Washington Seattle USA"},{"name":"Institute for Systems Biology  Washington Seattle USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan H. 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