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However, unlike GroEL, MtbCpn60.1 and MtbCpn60.2 purify as lower\u2010order oligomers. Previous studies have shown that MtbCpn60.2 can functionally replace GroEL in <jats:italic>E. coli<\/jats:italic>, while the function of MtbCpn60.1 remained an enigma. Here, we demonstrate the molecular chaperone function of MtbCpn60.1 and MtbCpn60.2, by probing their ability to assist the folding of obligate chaperonin clients, DapA, FtsE and MetK, in an <jats:italic>E. coli<\/jats:italic> strain depleted of endogenous GroEL. We show that both MtbCpn60.1 and MtbCpn60.2 support cell survival and cell division by assisting the folding of DapA and FtsE, but only MtbCpn60.2 completely rescues GroEL\u2010depleted <jats:italic>E. coli<\/jats:italic> cells. We also show that, unlike MtbCpn60.2, MtbCpn60.1 has limited ability to support cell growth and proliferation and assist the folding of MetK. Our findings suggest that the client pools of GroEL and MtbCpn60.2 overlap substantially, while MtbCpn60.1 folds only a small subset of GroEL clients. We conclude that the differences between MtbCpn60.1 and MtbCpn60.2 may be a consequence of their intrinsic sequence features, which affect their thermostability, efficiency, clientomes and modes of action.<\/jats:p>","DOI":"10.1111\/mmi.15109","type":"journal-article","created":{"date-parts":[[2023,6,24]],"date-time":"2023-06-24T23:58:51Z","timestamp":1687651131000},"page":"210-223","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Mycobacterial chaperonins in cellular proteostasis: Evidence for chaperone function of Cpn60.1 and Cpn60.2\u2010mediated protein folding"],"prefix":"10.1111","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7777-2154","authenticated-orcid":false,"given":"Bakul","family":"Piplani","sequence":"first","affiliation":[{"name":"Kusuma School of Biological Sciences Indian Institute of Technology Delhi  India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1370-2365","authenticated-orcid":false,"given":"C. M. Santosh","family":"Kumar","sequence":"additional","affiliation":[{"name":"School of Biosciences University of Birmingham  Birmingham"},{"name":"Institute of Microbiology and Infection University of Birmingham  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6381-8526","authenticated-orcid":false,"given":"Peter A.","family":"Lund","sequence":"additional","affiliation":[{"name":"School of Biosciences University of Birmingham  Birmingham"},{"name":"Institute of Microbiology and Infection University of Birmingham  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3668-1640","authenticated-orcid":false,"given":"Tapan K.","family":"Chaudhuri","sequence":"additional","affiliation":[{"name":"Kusuma School of Biological Sciences Indian Institute of Technology Delhi  India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,23]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378\u20101119(88)90440\u20104"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00102a010"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aac4354"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/371578a0"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01569\u201008"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01379\u201009"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607534103"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00844\u201015"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102958.1993.tb01624.x"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574\u20106968.12276"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102958.1992.tb01372.x"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.6.1497\u20101504.1995"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2003.10.051"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092\u20108674(00)80509\u20107"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102958.2012.08150.x"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.3.1379\u20101385.1989"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2010.52"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00493\u201007"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.055079\u20100"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstl.1825.0026"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00239\u2010006\u20100037\u20107"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1101\/SQB.1981.045.01.022"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1068408"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2015.07.009"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2010.01.004"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00143\u201009"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462\u20105822.2010.01496.x"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1256"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010biophys\u2010082521\u2010113418"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0033583519000143"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092\u20108674(93)90470\u2010B"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/45977"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01078\u201007"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20101119(97)00087\u20105"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1242\/bio.023119"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.05.028"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.7.2608"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2021.669996"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00652\u201009"},{"issue":"11","key":"e_1_2_12_41_1","first-page":"1646","article-title":"Protein 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