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Recently, we characterized T7SSb in group B\n                    <jats:italic>Streptococcus<\/jats:italic>\n                    (GBS), a leading cause of infection in newborns and immunocompromised adults. GBS T7SS comprises four subtypes based on variation in the C\u2010terminus of EssC and the repertoire of downstream effectors; however, the intraspecies diversity of GBS T7SS and impact on GBS\u2010host interactions remains unknown. Bioinformatic analysis indicates that GBS T7SS loci encode subtype\u2010specific putative effectors, which have low interspecies and inter\u2010subtype homology but contain similar domains\/motifs and therefore may serve similar functions. We further identify orphaned GBS WXG100 proteins. Functionally, we show that GBS T7SS subtype I and III strains secrete EsxA in vitro and that in subtype I strain CJB111,\n                    <jats:italic>esxA1<\/jats:italic>\n                    appears to be differentially transcribed from the T7SS operon. Furthermore, we observe subtype\u2010specific effects of GBS T7SS on host colonization, as CJB111 subtype I but not CNCTC 10\/84 subtype III T7SS promotes GBS vaginal colonization. Finally, we observe that T7SS subtypes I and II are the predominant subtypes in clinical GBS isolates. This study highlights the potential impact of T7SS heterogeneity on host\u2010GBS interactions\n                    <jats:italic>.<\/jats:italic>\n                  <\/jats:p>","DOI":"10.1111\/mmi.15115","type":"journal-article","created":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T05:09:34Z","timestamp":1687756174000},"page":"258-275","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Heterogeneity of the group B streptococcal type\n                    <scp>VII<\/scp>\n                    secretion system and influence on colonization of the female genital tract"],"prefix":"10.1111","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4374-6403","authenticated-orcid":false,"given":"Brady L.","family":"Spencer","sequence":"first","affiliation":[{"name":"Department of Immunology and Microbiology University of Colorado\u2010Anschutz  Aurora Colorado 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