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We have previously used C\u2010type lectin (CTL) pathogen receptors Dectin\u20101, Dectin\u20102, and DC\u2010SIGN to target DectiSomes to the extracellular oligoglycans surrounding diverse pathogenic fungi and kill them. Dectin\u20103 (also known as MCL, <jats:italic>CLEC4D<\/jats:italic>) is a CTL pathogen receptor whose known cognate ligands are partly distinct from other CTLs. We expressed and purified a truncated Dectin\u20103 polypeptide (DEC3) comprised of its carbohydrate recognition domain and stalk region. We prepared amphotericin B (AmB)\u2010loaded pegylated liposomes (AmB\u2010LLs) and coated them with this isoform of Dectin\u20103 (DEC3\u2010AmB\u2010LLs), and we prepared control liposomes coated with bovine serum albumin (BSA\u2010AmB\u2010LLs). DEC3\u2010AmB\u2010LLs bound to the exopolysaccharide matrices of <jats:italic>Candida albicans<\/jats:italic>, <jats:italic>Rhizopus delemar<\/jats:italic> (formerly known as <jats:italic>R. oryzae<\/jats:italic>), and <jats:italic>Cryptococcus neoformans<\/jats:italic> from one to several orders of magnitude more strongly than untargeted AmB\u2010LLs or BSA\u2010AmB\u2010LLs. The data from our quantitative fluorescent binding assays were standardized using a CellProfiler program, AreaPipe, that was developed for this purpose. Consistent with enhanced binding, DEC3\u2010AmB\u2010LLs inhibited and\/or killed <jats:italic>C. albicans<\/jats:italic> and <jats:italic>R. delemar<\/jats:italic> more efficiently than control liposomes and significantly reduced the effective dose of AmB. In conclusion, Dectin\u20103 targeting has the potential to advance our goal of building pan\u2010antifungal DectiSomes.<\/jats:p>","DOI":"10.1111\/mmi.15174","type":"journal-article","created":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T04:52:19Z","timestamp":1696654339000},"page":"723-739","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Dectin\u20103\u2010targeted antifungal liposomes efficiently bind and kill diverse fungal pathogens"],"prefix":"10.1111","volume":"120","author":[{"given":"Quanita J.","family":"Choudhury","sequence":"first","affiliation":[{"name":"Department of Microbiology University of Georgia  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh","family":"Ambati","sequence":"additional","affiliation":[{"name":"Department of Genetics University of Georgia  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Collin 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