{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T16:11:15Z","timestamp":1776615075114,"version":"3.51.2"},"reference-count":88,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T00:00:00Z","timestamp":1696636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["HA6830\/2\u20101"],"award-info":[{"award-number":["HA6830\/2\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Listeria monocytogenes<\/jats:italic> is a foodborne bacterium that naturally occurs in the soil. Originating from there, it contaminates crops and infects farm animals and their consumption by humans may lead to listeriosis, a systemic life\u2010threatening infectious disease. The adaptation of <jats:italic>L. monocytogenes<\/jats:italic> to such contrastive habitats is reflected by the presence of virulence genes for host infection and other genes for survival under environmental conditions. Among the latter are ABC transporters for excretion of antibiotics produced by environmental competitors; however, most of these transporters have not been characterized. Here, we generated a collection of promoter\u2010<jats:italic>lacZ<\/jats:italic> fusions for genes encoding ABC\u2010type drug transporters of <jats:italic>L. monocytogenes<\/jats:italic> and screened this reporter strain collection for induction using a library of natural compounds produced by various environmental microorganisms. We found that the <jats:italic>timABR<\/jats:italic> locus (<jats:italic>lmo1964\u2010lmo1962<\/jats:italic>) was induced by the macrodiolide antibiotic tartrolon B, which is synthesized by the soil myxobacterium <jats:italic>Sorangium cellulosum<\/jats:italic>. Tartrolon B resistance of <jats:italic>L. monocytogenes<\/jats:italic> was dependent on <jats:italic>timAB<\/jats:italic>, encoding the ATPase and the permease component of a novel ABC transporter. Moreover, transplantation of <jats:italic>timAB<\/jats:italic> was sufficient to confer tartrolon B resistance to <jats:italic>Bacillus subtilis<\/jats:italic>. Expression of the <jats:italic>timABR<\/jats:italic> locus was found to be auto\u2010repressed by the TimR repressor, whose repressing activity was lost in the presence of tartrolon B. We also demonstrate that tartrolon sensitivity was suppressed by high external potassium concentrations, suggesting that tartrolon acts as potassium ionophore. Our results help to map the ecological interactions of an important human pathogen with its co\u2010residing species within their joint natural reservoir.<\/jats:p>","DOI":"10.1111\/mmi.15178","type":"journal-article","created":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T18:43:07Z","timestamp":1696790587000},"page":"629-644","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Tartrolon sensing and detoxification by the <i>Listeria monocytogenes timABR<\/i> resistance operon"],"prefix":"10.1111","volume":"120","author":[{"given":"Tim","family":"Engelgeh","sequence":"first","affiliation":[{"name":"FG11 Division of Enteropathogenic Bacteria and Legionella Robert Koch Institute  Wernigerode Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Herrmann","sequence":"additional","affiliation":[{"name":"Department of Microbial Natural Products, Helmholtz Centre for Infection Research Saarland University  Saarbr\u00fccken Germany"},{"name":"Department of Pharmaceutical Biotechnology, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) Saarland University  Saarbr\u00fccken Germany"},{"name":"German Centre for Infection Research (DZIF) Partner Site Hannover\u2010Braunschweig  Braunschweig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolf","family":"Jansen","sequence":"additional","affiliation":[{"name":"Department of Microbial Drugs Helmholtz Centre for Infection Research  Braunschweig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolf","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Microbial Natural Products, Helmholtz Centre for Infection Research Saarland University  Saarbr\u00fccken Germany"},{"name":"Department of Pharmaceutical Biotechnology, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) Saarland University  Saarbr\u00fccken Germany"},{"name":"German Centre for Infection Research (DZIF) Partner Site Hannover\u2010Braunschweig  Braunschweig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5575-8973","authenticated-orcid":false,"given":"Sven","family":"Halbedel","sequence":"additional","affiliation":[{"name":"FG11 Division of Enteropathogenic Bacteria and Legionella Robert Koch Institute  Wernigerode Germany"},{"name":"Institute for Medical Microbiology and Hospital Hygiene Otto von Guericke University Magdeburg  Magdeburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,10,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0168\u20101605(00)00357\u20103"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes11111330"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.11.6887\u20106891.2004"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751\u20107915.13926"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2007.0019"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02700"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2014.11.001"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473\u20103099(16)30521\u20107"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/415545a"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00503\u201010"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0804170105"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.052720\u20100"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362\u2010028X\u201055.12.1009"},{"key":"e_1_2_9_15_1","unstructured":"DZIF\/TTU9. (2023)Compound resources and medicinal chemistry.https:\/\/www.dzif.de\/en\/compound\u2010resources\u2010and\u2010medicinal\u2010chemistry"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1213892110"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268805005601"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0079\u20106603(03)75008\u20109"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746\u20106148\u20105\u20102"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2171"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2014.00009"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioadv\/vbab016"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1063447294\/5543\/849"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.2.704\u2010708.2003"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.48.119"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0302\u20104598(94)01721\u2010C"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00901\u201017"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0106340"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00861\u201017"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00039\u201018"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201027069\u2010y"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkf698"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00503\u201020"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14205"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2018.00057"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/hlca.19670500612"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.48.26"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.1401"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01470\u201019"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00794\u201013"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2010.0054"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00772"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432\u20101033.1988.tb14344.x"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1039\/b613652b"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2008.11.012"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2022.2063075"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.738470"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(98)00580-0"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00001\u201007"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010007\u20107171\u20102"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb09158.x"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00314\u201008"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00199"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574\u20106968.12626"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.1c00717"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2022.116986"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168\u20101605(92)90034\u2010z"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010016\u20107552\u20102"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00409681"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1021\/np9006603"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1039\/c8sc04396e"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2021.1975526"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00259\u201017"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl925"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh016"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00956\u201006"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2019.10.016"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01193\u201015"},{"key":"e_1_2_9_69_1","first-page":"3","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.15666\/aeer\/1904_30693081"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102672.2006.02964.x"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods11213472"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlac.199419940310"},{"key":"e_1_2_9_74_1","first-page":"506","article-title":"Survival of listeria monocytogenes in high salt concentrations","volume":"246","author":"Shahamat M.","year":"1980","journal-title":"Zentralbl Bakteriol A"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102958.2004.04454.x"},{"key":"e_1_2_9_76_1","first-page":"61","volume-title":"Metagenomics and its applications in agriculture, biomedicine and environmental studies","author":"Solovyev V.","year":"2011"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000808"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08080"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.1255"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbbm.2005.12.008"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.3.584\u2010640.2001"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12275\u2010011\u20101260\u20105"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2013.00087"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2014.10.002"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2709.210068"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1189801"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1038\/msb.2012.11"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.29043\u20100"},{"key":"e_1_2_9_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0005\u20102736(99)00158\u20103"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.15178","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,11]],"date-time":"2023-11-11T08:49:49Z","timestamp":1699692589000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.15178"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,7]]},"references-count":88,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,11]]}},"alternative-id":["10.1111\/mmi.15178"],"URL":"https:\/\/doi.org\/10.1111\/mmi.15178","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,7]]},"assertion":[{"value":"2023-07-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}