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Despite evidence indicating that <jats:italic>Fusarium oxysporum<\/jats:italic> organizes itself in the form of a biofilm causing onychomycosis, there is little literature on the etiopathogenesis of the biofilm on the nail, specifically the signaling molecules present, known as <jats:italic>quorum sensing<\/jats:italic> (QS). Thus, this study detected the presence of a molecule related to QS from the ex\u00a0vivo biofilm of <jats:italic>F. oxysporum<\/jats:italic> on human nail and investigated its effect on preformed biofilm in\u00a0vitro. The detection and physicochemical characterization of a QS molecule, from the extracellular matrix (ECM), was carried out by Fourier transform infrared (FTIR) spectroscopy with an attenuated total reflectance (ATR) accessory and by headspace gas chromatography coupled to mass spectrometry (GC\u2013MS) analyses. Determination of viable cells, cell activity, total biomass, ECM components and scanning electron microscopy (SEM) were performed to evaluate the influence of the QS molecule on the in\u00a0vitro biofilm of <jats:italic>F. oxysporum<\/jats:italic>. The beginning, in the ex\u00a0vivo biofilm of <jats:italic>F. oxysporum<\/jats:italic> on human nails, the volatile organic compound 2\u2010ethyl\u20101\u2010hexanol (2EH) was detected as a component of QS. Thereafter in\u00a0vitro analyses, synthetic 2EH was able to modulate the biofilm by stimulating its filament, increasing total biomass and ECM production in terms of total carbohydrates, but with a reduction in total proteins and nucleic acids. We thus evidence, for the first time, the presence of 2EH in the biofilm of <jats:italic>F. oxysporum<\/jats:italic>, developed on the human nail, and the in\u00a0vitro action of this compound as a QS molecule.<\/jats:p>","DOI":"10.1111\/mmi.15194","type":"journal-article","created":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T13:59:21Z","timestamp":1701439161000},"page":"630-642","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Detection of 2\u2010ethyl\u20101\u2010hexanol and its modulating effect in biofilm of <i>Fusarium oxysporum<\/i>"],"prefix":"10.1111","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5562-0811","authenticated-orcid":false,"given":"Flavia Franco","family":"Veiga","sequence":"first","affiliation":[{"name":"Clinical Analysis Department Universidade Estadual de Maring\u00e1  Maring\u00e1 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