{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T03:10:39Z","timestamp":1773889839259,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,11]],"date-time":"2023-12-11T00:00:00Z","timestamp":1702252800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2023,12,11]],"date-time":"2023-12-11T00:00:00Z","timestamp":1702252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI149124"],"award-info":[{"award-number":["AI149124"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DMR\u20101933525"],"award-info":[{"award-number":["DMR\u20101933525"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Brucella abortus<\/jats:italic> is a facultative, intracellular, zoonotic pathogen that resides inside macrophages during infection. This is a specialized niche where <jats:italic>B. abortus<\/jats:italic> encounters various stresses as it navigates through the macrophage. In order to survive this harsh environment, <jats:italic>B. abortus<\/jats:italic> utilizes post\u2010transcriptional regulation of gene expression through the use of small regulatory RNAs (sRNAs). Here, we characterize a <jats:italic>Brucella<\/jats:italic> sRNAs called MavR (for <jats:italic>M<\/jats:italic>urF\u2010 <jats:italic>a<\/jats:italic>nd <jats:italic>v<\/jats:italic>irulence\u2010regulating s<jats:italic>R<\/jats:italic>NA), and we demonstrate that MavR is required for the full virulence of <jats:italic>B. abortus<\/jats:italic> in macrophages and in a mouse model of chronic infection. Transcriptomic and proteomic studies revealed that a major regulatory target of MavR is MurF. MurF is an essential protein that catalyzes the final cytoplasmic step in peptidoglycan (PG) synthesis; however, we did not detect any differences in the amount or chemical composition of PG in the \u0394<jats:italic>mavR<\/jats:italic> mutant. A 6\u2010nucleotide regulatory seed region within MavR was identified, and mutation of this seed region resulted in dysregulation of MurF production, as well as significant attenuation of infection in a mouse model. Overall, the present study underscores the importance of sRNA regulation in the physiology and virulence of <jats:italic>Brucella<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/mmi.15207","type":"journal-article","created":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T19:56:19Z","timestamp":1702410979000},"page":"129-141","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Post\u2010transcriptional control of the essential enzyme <scp>MurF<\/scp> by a small regulatory <scp>RNA<\/scp> in <i>Brucella abortus<\/i>"],"prefix":"10.1111","volume":"121","author":[{"given":"Kellie A.","family":"King","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology, Center for One Health Research, VA\u2010MD College of Veterinary Medicine Virginia Tech  Blacksburg Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela H.","family":"Benton","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology, Center for One Health Research, VA\u2010MD College of Veterinary Medicine Virginia Tech  Blacksburg Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitchell T.","family":"Caudill","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology, Center for One Health Research, VA\u2010MD College of Veterinary Medicine Virginia Tech  Blacksburg Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. Tristan","family":"Stoyanof","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology, Center for One Health Research, VA\u2010MD College of Veterinary Medicine Virginia Tech  Blacksburg Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Edward Via College of Osteopathic Medicine  Monroe Louisiana USA"},{"name":"College of Pharmacy University of Louisiana Monroe  Monroe Louisiana USA"},{"name":"Center for One Health Research Edward Via College of Osteopathic Medicine  Blacksburg Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pawel","family":"Michalak","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Edward Via College of Osteopathic Medicine  Monroe Louisiana USA"},{"name":"Center for One Health Research Edward Via College of Osteopathic Medicine  Blacksburg Virginia USA"},{"name":"Institute for Evolution University of Haifa  Haifa Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin K.","family":"Lahmers","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology, Center for One Health Research, VA\u2010MD College of Veterinary Medicine Virginia Tech  Blacksburg Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul M.","family":"Dunman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Rochester Medical Center  Rochester New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanner G.","family":"DeHart","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Virginia Tech  Blacksburg Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saadman S.","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Virginia Tech  Blacksburg Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brandon L.","family":"Jutras","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Virginia Tech  Blacksburg Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katy","family":"Poncin","sequence":"additional","affiliation":[{"name":"URBM, Narilis University of Namur  Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3845-3043","authenticated-orcid":false,"given":"Xavier","family":"De\u00a0Bolle","sequence":"additional","affiliation":[{"name":"URBM, Narilis University of Namur  Namur Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3793-8804","authenticated-orcid":false,"given":"Clayton 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USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,12,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1875036201307010001"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12223-019-00691-6"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2008.00104.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.14.4665-4684.2004"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2012.52"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep22372"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00127-18"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2012.08117.x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05623-11"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.BAI-0006-2019"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030088"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009546"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-021-01003-w"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2012.10.004"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02173"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6968.12433"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9701078"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.RWR\u20100022\u20102018"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/br.22.2.81-98.1958"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00214-6"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20140236"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20160363"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(03)00167-7"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1904170116"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.1026220"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12758"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp324"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.11.6126-6134.1989"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00213"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10050942"},{"key":"e_1_2_10_32_1","doi-asserted-by":"crossref","DOI":"10.1128\/jcm.00438-23","article-title":"If 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