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Once fully matured, gametocytes are able to transform into gametes in the mosquito's midgut, a process accompanied with their egress from the enveloping erythrocyte. Gametocyte maturation and gametogenesis require a well\u2010coordinated gene expression program that involves a wide spectrum of regulatory proteins, ranging from histone modifiers to transcription factors to RNA\u2010binding proteins. Here, we investigated the role of the CCCH zinc finger protein MD3 in <jats:italic>Plasmodium falciparum<\/jats:italic> gametocytogenesis. MD3 was originally identified as an epigenetically regulated protein of immature gametocytes and recently shown to be involved in male development in a barcode\u2010based screen in <jats:italic>P. berghei<\/jats:italic>. We report that MD3 is mainly present in the cytoplasm of immature male <jats:italic>P. falciparum<\/jats:italic> gametocytes. Parasites deficient of MD3 are impaired in gametocyte maturation and male gametocytogenesis. BioID analysis in combination with co\u2010immunoprecipitation assays unveiled an interaction network of MD3 with RNA\u2010binding proteins like PABP1 and ALBA3, with translational initiators, regulators and repressors like elF4G, PUF1, NOT1 and CITH, and with further regulators of gametocytogenesis, including ZNF4, MD1 and GD1. We conclude that MD3 is part of a regulator complex crucial for post\u2010transcriptional fine\u2010tuning of male gametocytogenesis.<\/jats:p>","DOI":"10.1111\/mmi.15215","type":"journal-article","created":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T06:00:08Z","timestamp":1703656808000},"page":"543-564","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The <i>Plasmodium falciparum<\/i><scp>CCCH<\/scp> zinc finger protein <scp>MD3<\/scp> regulates male gametocytogenesis through its interaction with <scp>RNA<\/scp>\u2010binding proteins"],"prefix":"10.1111","volume":"121","author":[{"given":"Afia","family":"Farrukh","sequence":"first","affiliation":[{"name":"Division of Cellular and Applied Infection Biology, Institute of Zoology RWTH Aachen University  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean 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