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Among others, the alternative sigma factor RpoS activates response pathways, including regulation of motility\u2010 and chemotaxis\u2010related genes under nutrient\u2010poor conditions in <jats:italic>V. cholerae<\/jats:italic>. Although RpoS has been well characterised, links between RpoS and other regulatory networks remain unclear. In this study, we identified the ArcAB two\u2010component system to control <jats:italic>rpoS<\/jats:italic> transcription and RpoS protein stability in <jats:italic>V. cholerae<\/jats:italic>. In a manner similar to that seen in <jats:italic>Escherichia coli<\/jats:italic>, the ArcB kinase not only activates the response regulator ArcA but also RssB, the anti\u2010sigma factor of RpoS. Our results demonstrated that, in <jats:italic>V. cholerae<\/jats:italic>, RssB is phosphorylated by ArcB, which subsequently activates RpoS proteolysis. Furthermore, ArcA acts as a repressor of <jats:italic>rpoS<\/jats:italic> transcription. Additionally, we determined that the cysteine residue at position 180 of ArcB is crucial for signal recognition and activity. Thus, our findings provide evidence linking RpoS response to the anoxic redox control system ArcAB in <jats:italic>V. cholerae<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/mmi.15235","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T10:55:04Z","timestamp":1707303304000},"page":"850-864","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The regulatory network comprising ArcAB\u2010RpoS\u2010RssB influences motility in <i>Vibrio cholerae<\/i>"],"prefix":"10.1111","volume":"121","author":[{"given":"Martina","family":"W\u00f6lflingseder","sequence":"first","affiliation":[{"name":"Institute of Molecular Biosciences, University of Graz  Graz Austria"},{"name":"BioTechMed\u2010Graz  Graz Austria"},{"name":"Field of Excellence BioHealth \u2013 University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera 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