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One of the principal membrane components is oleic acid, which is needed to maintain the membrane's biophysical properties and fluidity. The malaria parasite can modify fatty acids, and stearoyl\u2010CoA \u03949\u2010desaturase (Scd) is an enzyme that catalyzes the synthesis of oleic acid by desaturation of stearic acid. Scd is dispensable in <jats:italic>P. falciparum<\/jats:italic> blood stages; however, its role in mosquito and liver stages remains unknown. We show that <jats:italic>P. berghei<\/jats:italic> Scd localizes to the ER in the blood and liver stages. Disruption of <jats:italic>Scd<\/jats:italic> in the rodent malaria parasite <jats:italic>P. berghei<\/jats:italic> did not affect parasite blood stage propagation, mosquito stage development, or early liver\u2010stage development. However, when <jats:italic>Scd<\/jats:italic> KO sporozoites were inoculated intravenously or by mosquito bite into mice, they failed to initiate blood\u2010stage infection. Immunofluorescence analysis revealed that organelle biogenesis was impaired and merozoite formation was abolished, which initiates blood\u2010stage infections. Genetic complementation of the KO parasites restored merozoite formation to a level similar to that of WT parasites. Mice immunized with <jats:italic>Scd<\/jats:italic> KO sporozoites confer long\u2010lasting sterile protection against infectious sporozoite challenge. Thus, the <jats:italic>Scd<\/jats:italic> KO parasite is an appealing candidate for inducing protective pre\u2010erythrocytic immunity and hence its utility as a GAP.<\/jats:p>","DOI":"10.1111\/mmi.15246","type":"journal-article","created":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T05:45:17Z","timestamp":1709185517000},"page":"940-953","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["<scp>Stearoyl\u2010CoA<\/scp> desaturase regulates organelle biogenesis and hepatic merozoite formation in <i>Plasmodium berghei<\/i>"],"prefix":"10.1111","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5476-9753","authenticated-orcid":false,"given":"Sunil Kumar","family":"Narwal","sequence":"first","affiliation":[{"name":"Division of Molecular Microbiology and Immunology CSIR\u2010Central Drug Research Institute  Lucknow 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