{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T19:30:11Z","timestamp":1767900611523,"version":"3.49.0"},"reference-count":88,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T00:00:00Z","timestamp":1714089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["BT\/PR13522\/CoE\/34\/27\/2015"],"award-info":[{"award-number":["BT\/PR13522\/CoE\/34\/27\/2015"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Latent tuberculosis, caused by dormant <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (<jats:italic>Mtb<\/jats:italic>), poses a threat to global health through the incubation of undiagnosed infections within the community. Dormant <jats:italic>Mtb<\/jats:italic>, which is phenotypically tolerant to antibiotics, accumulates triacylglycerol (TAG) utilizing fatty acids obtained from macrophage lipid droplets. TAG is vital to mycobacteria, serving as a cell envelope component and energy reservoir during latency. TAG synthesis occurs by sequential acylation of glycerol\u20103\u2010phosphate, wherein the second acylation step is catalyzed by acylglycerol\u20103\u2010phosphate acyltransferase (AGPAT), resulting in the production of phosphatidic acid (PA), a precursor for the synthesis of TAG and various phospholipids. Here, we have characterized a putative acyltransferase of <jats:italic>Mtb<\/jats:italic> encoded by Rv3816c. We found that Rv3816c has all four characteristic motifs of AGPAT, exists as a membrane\u2010bound enzyme, and functions as 1\u2010acylglycerol\u20103\u2010phosphate acyltransferase. The enzyme could transfer the acyl group to acylglycerol\u20103\u2010phosphate (LPA) from monounsaturated fatty acyl\u2010coenzyme A of chain length 16 or 18 to produce PA. Complementation of <jats:italic>Escherichia coli<\/jats:italic> PlsC mutant in\u00a0vivo by Rv3816c confirmed that it functions as AGPAT. Its active site mutants, H43A and D48A, were incapable of transferring the acyl group to LPA in\u00a0vitro and were not able to rescue the growth defect of <jats:italic>E. coli<\/jats:italic> PlsC mutant in\u00a0vivo. Identifying Rv3816c as AGPAT and comparing its properties with other AGPAT homologs is not only a step toward understanding the TAG biosynthesis in mycobacteria but has the potential to explore it as a drug target.<\/jats:p>","DOI":"10.1111\/mmi.15265","type":"journal-article","created":{"date-parts":[[2024,4,27]],"date-time":"2024-04-27T06:54:30Z","timestamp":1714200870000},"page":"1164-1181","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Identification of a 1\u2010acyl\u2010glycerol\u20103\u2010phosphate acyltransferase from <i>Mycobacterium tuberculosis<\/i>, a key enzyme involved in triacylglycerol biosynthesis"],"prefix":"10.1111","volume":"121","author":[{"given":"Meghna","family":"Santoshi","sequence":"first","affiliation":[{"name":"Department of Microbiology and Cell Biology Indian Institute of Science  Bengaluru India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harsh","family":"Bansia","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Indian Institute of Science  Bengaluru India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muzammil","family":"Hussain","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Indian Institute of Science  Bengaluru India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abodh Kumar","family":"Jha","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Indian Institute of Science  Bengaluru India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9711-5933","authenticated-orcid":false,"given":"Valakunja","family":"Nagaraja","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Indian Institute of Science  Bengaluru India"},{"name":"Jawaharlal Nehru Centre for Advanced Scientific Research  Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,26]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"164","article-title":"The granulomatous inflammatory response. A review","volume":"84","author":"Adams D.O.","year":"1976","journal-title":"The American Journal of Pathology"},{"key":"e_1_2_10_3_1","first-page":"7","article-title":"Structural basis for selective recognition of acyl chains by the membrane\u2010associated acyltransferase PatA","author":"Albesa\u2010Jov\u00e9 D.","year":"2016","journal-title":"Nature Communications"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.001058"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-42478-x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abj4565"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00822.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01121-07"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001065"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M406796200"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.120.3.913"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00043651"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00039533"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)54770-0"},{"key":"e_1_2_10_15_1","first-page":"92","article-title":"Characterization of 1\u2010acyl\u2010sn\u2010glycerol\u20103\u2010phosphate acyltransferase from a polyunsaturated fatty acid\u2010producing bacterium, Shewanella livingstonensis Ac10","volume":"29","author":"Cho H.\u2010N.","year":"2012","journal-title":"Trace Nutrients Research"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/31159"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)44891-5"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00280009"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad896"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-3879(51)80038-2"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/ecosalplus.3.6.4"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-92721-y"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27958-0"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.MGM2-0021-2013"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.15.5017-5030.2004"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002093"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M505556200"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006077"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02133-16"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7827(99)00012-0"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.49.34728"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.1c00203"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10061558"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-10-2951"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0050075"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2012.00331.x"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02081-12"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1915"},{"key":"e_1_2_10_39_1","unstructured":"Global Tuberculosis Report. (2022)https:\/\/www.who.int\/publications\/i\/item\/9789240061729."},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2003.08.003"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002251"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202002591R"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1995.0806a.x"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2009.09.006"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.6.1425-1430.1998"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M500435-JLR200"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02531442"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00530-09"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053657"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2010.09.006"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.3.999"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btaa579"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.7214"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)70579-1"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.10.050"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi982805d"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz268"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00450"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/35021074"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.1.546-551.2005"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)80661-9"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvy025"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)45574-9"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)43689-2"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000204"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M311209200"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M007575"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku316"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.3436"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(02)00006-6"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2134250100"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01404.x"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2012.09.012"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28993-0"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3260455"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1677\/JME-09-0010"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.mmi_18030401.x"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.90958.2008"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.7b00578"},{"key":"e_1_2_10_81_1","unstructured":"The PyMOL Molecular Graphics System Version 2.0 Schr\u00f6dinger LLC."},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.1038\/227138a0"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.04.140"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.364851"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21334"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04441.x"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.6.2062-2069.1996"},{"key":"e_1_2_10_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2007.07.002"},{"key":"e_1_2_10_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2012.08.018"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.15265","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T03:53:18Z","timestamp":1718250798000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.15265"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,26]]},"references-count":88,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1111\/mmi.15265"],"URL":"https:\/\/doi.org\/10.1111\/mmi.15265","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,26]]},"assertion":[{"value":"2023-12-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}