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Building on this progress, we engineered the <jats:italic>T. pallidum<\/jats:italic> SS14 strain to express a red\u2010shifted green fluorescent protein (GFP) and Sf1Ep cells to express mCherry and blue fluorescent protein (BFP) for enhanced visualization. These new resources improve microscopy\u2010 and cell sorting\u2013based applications for <jats:italic>T. pallidum<\/jats:italic>, better capturing the physical interaction between the host and pathogen, among other possibilities. Continued efforts to develop and share new tools and resources are required to help our overall knowledge of <jats:italic>T. pallidum<\/jats:italic> biology and syphilis pathogenesis reach that of other bacterial pathogens, including spirochetes.<\/jats:p>","DOI":"10.1111\/mmi.15304","type":"journal-article","created":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T10:29:19Z","timestamp":1723112959000},"page":"455-464","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Bright New Resources for Syphilis Research: Genetically Encoded Fluorescent Tags for <i>Treponema pallidum<\/i> and <scp>Sf1Ep<\/scp> Cells"],"prefix":"10.1111","volume":"122","author":[{"given":"Linda","family":"Grillov\u00e1","sequence":"first","affiliation":[{"name":"Parasites and Microbes Programme Wellcome Sanger Institute  Hinxton UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Romeis","sequence":"additional","affiliation":[{"name":"Division of Allergy and Infectious Diseases, Department of Medicine University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole\u00a0A.\u00a0P.","family":"Lieberman","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren\u00a0C.","family":"Tantalo","sequence":"additional","affiliation":[{"name":"Division of Allergy and Infectious Diseases, Department of Medicine University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda\u00a0H.","family":"Xu","sequence":"additional","affiliation":[{"name":"Division of Allergy and Infectious Diseases, Department of Medicine University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Molini","sequence":"additional","affiliation":[{"name":"Division of Allergy and Infectious Diseases, Department of Medicine University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldo\u00a0T.","family":"Trejos","sequence":"additional","affiliation":[{"name":"Division of Allergy and Infectious Diseases, Department of Medicine University of Washington  Seattle Washington USA"},{"name":"Department of Global Health University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Lacey","sequence":"additional","affiliation":[{"name":"Parasites and Microbes Programme Wellcome Sanger Institute  Hinxton UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Goulding","sequence":"additional","affiliation":[{"name":"Parasites and Microbes Programme Wellcome Sanger Institute  Hinxton UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas\u00a0R.","family":"Thomson","sequence":"additional","affiliation":[{"name":"Parasites and Microbes Programme Wellcome Sanger Institute  Hinxton UK"},{"name":"Faculty of Infectious and Tropical Diseases London School of Hygiene &amp; 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