{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T13:17:13Z","timestamp":1776259033824,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,12,25]],"date-time":"2024-12-25T00:00:00Z","timestamp":1735084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM70641"],"award-info":[{"award-number":["GM70641"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001381","name":"National Research Foundation Singapore","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001381","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["ES026856"],"award-info":[{"award-number":["ES026856"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["ES031529"],"award-info":[{"award-number":["ES031529"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Queuosine (Q) is a modification of the wobble base in tRNAs that decode NA(C\/U) codons. It is ubiquitous in bacteria, including many pathogens. <jats:styled-content style=\"fixed-case\"><jats:italic>Streptococcus mutans<\/jats:italic><\/jats:styled-content> is an early colonizer of dental plaque biofilm and a key player in dental caries. Using a combination of genetic and physiological approaches, the predicted <jats:italic>Q<\/jats:italic> synthesis and salvage pathways were validated in this organism. These experiments confirmed that <jats:styled-content style=\"fixed-case\"><jats:italic>S. mutans<\/jats:italic><\/jats:styled-content> can synthesize <jats:italic>Q de novo<\/jats:italic> through similar pathways found in <jats:styled-content style=\"fixed-case\"><jats:italic>Bacillus subtilis<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>Escherichia coli<\/jats:italic><\/jats:styled-content>. However, <jats:styled-content style=\"fixed-case\"><jats:italic>S. mutans<\/jats:italic><\/jats:styled-content> has a distinct salvage pathway compared to these model organisms, as it uses a transporter belonging to the energy coupling factor (ECF) family controlled by a preQ<jats:sub>1<\/jats:sub>\u2010dependent riboswitch. Furthermore, Q levels in this oral pathogen depended heavily on the media composition, suggesting that micronutrients can affect Q\u2010mediated translation efficiency.<\/jats:p>","DOI":"10.1111\/mmi.15336","type":"journal-article","created":{"date-parts":[[2024,12,25]],"date-time":"2024-12-25T08:14:47Z","timestamp":1735114487000},"page":"48-59","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Environmental Control of Queuosine Levels in <i>Streptococcus mutans<\/i><scp>tRNAs<\/scp>"],"prefix":"10.1111","volume":"123","author":[{"given":"Marshall","family":"Jaroch","sequence":"first","affiliation":[{"name":"Department of Microbiology and Cell Science, IFAS University of Florida  Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathryn","family":"Savage","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, IFAS University of Florida  Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Kuipers","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, IFAS University of Florida  Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo Marie","family":"Bacusmo","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, IFAS University of Florida  Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering Massachusetts Institute of Technology  Cambridge Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjing","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering Massachusetts Institute of Technology  Cambridge Massachusetts USA"},{"name":"Antimicrobial Resistance Interdisciplinary Research Group Singapore\u2010MIT Alliance for Research and Technology  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter C.","family":"Dedon","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering Massachusetts Institute of Technology  Cambridge Massachusetts USA"},{"name":"Antimicrobial Resistance Interdisciplinary Research Group Singapore\u2010MIT Alliance for Research and Technology  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1335-4409","authenticated-orcid":false,"given":"Kelly C.","family":"Rice","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, IFAS University of Florida  Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9955-3785","authenticated-orcid":false,"given":"Val\u00e9rie","family":"de Cr\u00e9cy\u2010Lagard","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, IFAS University of Florida  Gainesville Florida USA"},{"name":"Genetics Institute University of Florida  Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2017.1356562"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.039586-0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.3.1631-1642.2006"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-024-01238-1"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.43.2.555-560.1984"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2017.1353861"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.4061\/2011\/408053"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0159599"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.4.2493-2501.2001"},{"key":"e_1_2_8_11_1","doi-asserted-by":"crossref","DOI":"10.1128\/mmbr.00199-23","article-title":"Biosynthesis and Function of 7\u2010Deazaguanine Derivatives in bacteria and Phages","volume":"88","author":"Cr\u00e9cy\u2010Lagard V.","year":"2024","journal-title":"Microbiology and Molecular Biology Reviews"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00337-12"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1204"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.96317.3"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010477"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.11.052"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/13.19.6881"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00764-19"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00624-12"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(01)00369-4"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.GPP3\u20100051\u20102018"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02145-08"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.5.1374-1382.2000"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1506"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2014.05.015"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.937308"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1018636108"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz063"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-47243-0"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01395-07"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-745"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1003"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb200329f"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/mtomcs\/mfac065"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/epigenomes8020016"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.001490"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310858200"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005353"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb1224"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1112"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01257-10"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00508-17"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00031-18"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034511415415"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2012.08187.x"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.047"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-021-00342-0"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.11.4.649-655.1975"},{"key":"e_1_2_8_50_1","unstructured":"ThermoFisher Scientific.2019.\u201cTechnical Guide to Peptones Supplements and Feeds. Enhancing Performance of Mammalian and Microbial Bioprocesses.\u201dhttps:\/\/assets.thermofisher.com\/TFS\u2010Assets\/BPD\/brochures\/peptones\u2010supplements\u2010feeds\u2010technical\u2010reference\u2010guide.pdf."},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.14.4296"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201899777"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00102-16"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2021.167073"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06403.x"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01403-17"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05359.x"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.075556.120"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.5.1100"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.15336","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T20:11:38Z","timestamp":1736539898000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.15336"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,25]]},"references-count":58,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/mmi.15336"],"URL":"https:\/\/doi.org\/10.1111\/mmi.15336","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,25]]},"assertion":[{"value":"2024-08-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}