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The Golgi in <jats:italic>Plasmodium<\/jats:italic> malaria parasites is composed of unstacked cisternae that contain a single GRASP homolog. We previously showed that the initial development of <jats:italic>Plasmodium berghei<\/jats:italic> in hepatocytes involves the clearance of micronemes through their sequestration into PbATG8\u2010positive autophagosomes that fuse with the parasite plasma membrane. Here, we examine the involvement of PbGRASP in microneme elimination and extend our studies to assess the importance of GRASP for parasite development in the mammalian host and mosquito vector. GRASP associates with PbATG8 autophagosomes containing micronemes, though PbGRASP\u2010KO parasites can expel micronemes. PbGRASP\u2010KO parasites have no discernable phenotype during mosquito stage development or asexual blood stage growth. PbGRASP\u2010KO liver stages form small schizonts at mid\u2010infection, and then growth resumes. PbGRASP\u2010KO hepatic merozoites egress from the mouse liver and induce higher parasitemia but display delayed and reduced cerebral malaria symptoms. These observations point to a regulatory role for GRASP in controlling parasite proliferation and virulence in mammalian hosts.<\/jats:p>","DOI":"10.1111\/mmi.15360","type":"journal-article","created":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T17:37:27Z","timestamp":1743269847000},"page":"487-515","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Plasmodium GRASP Homolog Modulates Liver Stage Development, Subsequent Blood Infection and Virulence in Mice"],"prefix":"10.1111","volume":"123","author":[{"given":"Ella J.","family":"Gehrke","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology and Immunology Johns Hopkins Malaria Research Institute, Johns Hopkins University Bloomberg School of Public Health  Baltimore Maryland 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