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These resistances can arise due to mutations through the activity of error\u2010prone DNA polymerases, such as DNA polymerase V (DNA Pol V) in response to DNA damage. The regulation of the DNA damage response (DDR) in <jats:styled-content style=\"fixed-case\"><jats:italic>A. baumannii<\/jats:italic><\/jats:styled-content> is not completely understood; the regulation of genes encoding multiple copies of DNA Pol V is not fully characterized. Through genome\u2010wide mutagenesis, we have identified a novel TetR\u2010like family regulator of the <jats:italic>umuDC<\/jats:italic> and <jats:italic>umuC<\/jats:italic> genes, which we have named Error\u2010prone polymerase regulator (EppR). We have found that EppR represses the expression of the genes encoding DNA Pol V and itself through direct binding to an EppR motif in their promoters. Lastly, we show that EppR also regulates UmuDAb, previously identified as a regulator of genes encoding DNA Pol V. These two gene products are functionally required to ensure regulation of the expression of the two <jats:italic>umuDC<\/jats:italic>, the two <jats:italic>umuC<\/jats:italic> genes as well as the regulators <jats:italic>umuDAb<\/jats:italic> and <jats:italic>eppR<\/jats:italic> genes. With these results, we propose a model in which multiple transcription factors regulate the expression of all these genes.<\/jats:p>","DOI":"10.1111\/mmi.15368","type":"journal-article","created":{"date-parts":[[2025,4,19]],"date-time":"2025-04-19T03:40:21Z","timestamp":1745034021000},"page":"20-39","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Identification of <scp>EppR<\/scp>, a Second Repressor of Error\u2010Prone <scp>DNA<\/scp> Polymerase Genes in <scp><i>Acinetobacter baumannii<\/i><\/scp>"],"prefix":"10.1111","volume":"124","author":[{"given":"Brian","family":"Nguyen","sequence":"first","affiliation":[{"name":"Northeastern University  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6785-636X","authenticated-orcid":false,"given":"Carly","family":"Ching","sequence":"additional","affiliation":[{"name":"Northeastern University  Boston 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