{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:19:34Z","timestamp":1760059174592,"version":"build-2065373602"},"reference-count":64,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T00:00:00Z","timestamp":1752710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Candida dubliniensis<\/jats:italic> is the most closely related species to <jats:styled-content style=\"fixed-case\"><jats:italic>C. albicans<\/jats:italic><\/jats:styled-content>, one of the leading causes of fungal infections in humans. However, despite sharing many characteristics, <jats:italic>C. dubliniensis<\/jats:italic> is significantly less pathogenic. To better understand the molecular underpinnings of these dissimilarities, we focused on the regulation of filamentation, a developmental trait fundamental for host colonization. We generated a collection of 44 <jats:italic>C. dubliniensis<\/jats:italic> null mutants of transcription regulators whose orthologs in <jats:styled-content style=\"fixed-case\"><jats:italic>C. albicans<\/jats:italic><\/jats:styled-content> had been previously implicated in filamentous growth. These regulators are very similar at the sequence level, but phenotypic screening identified several mutants with contrasting interspecific filamentation phenotypes beyond previously known differences. Bcr1, a well\u2010known regulator of biofilm formation, stands out as its mutant mainly showed a filamentation defect in <jats:italic>C. dubliniensis<\/jats:italic>. Phenotypic and transcriptional characterization showed that the <jats:italic>bcr1<\/jats:italic> defect is condition dependent and that this regulator plays a central role in the filamentation of <jats:italic>C. dubliniensis<\/jats:italic>, possibly by regulating the hyphal activator Ume6. Overall, our results suggest that several regulatory pathways are involved in the filamentation differences between <jats:styled-content style=\"fixed-case\"><jats:italic>C. albicans<\/jats:italic><\/jats:styled-content> and <jats:italic>C. dubliniensis<\/jats:italic> and show that the <jats:italic>C. dubliniensis<\/jats:italic> mutant collection is a valuable resource to compare, at a molecular level, these species of medical relevance.<\/jats:p>","DOI":"10.1111\/mmi.70012","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T10:04:21Z","timestamp":1752746661000},"page":"327-341","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Filamentation Profiling Reveals Multiple Transcription Regulators Contributing to the Differences Between <i>Candida albicans<\/i> and <i>Candida dubliniensis<\/i>"],"prefix":"10.1111","volume":"124","author":[{"given":"Teresa","family":"Meza\u2010Davalos","sequence":"first","affiliation":[{"name":"Departamento de Ingenier\u00eda Gen\u00e9tica Unidad Irapuato, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional  Irapuato Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2021-7165","authenticated-orcid":false,"given":"Luis F.","family":"Garc\u00eda\u2010Ortega","sequence":"additional","affiliation":[{"name":"Departamento de Ingenier\u00eda Gen\u00e9tica Unidad Irapuato, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional  Irapuato Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0146-8732","authenticated-orcid":false,"given":"Eugenio","family":"Mancera","sequence":"additional","affiliation":[{"name":"Departamento de Ingenier\u00eda Gen\u00e9tica Unidad Irapuato, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional  Irapuato Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,17]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"Alexa A.2024.\u201cTopgo: Enrichment Analysis for Gene Ontology. 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