{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:19:27Z","timestamp":1760059167108,"version":"build-2065373602"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T00:00:00Z","timestamp":1752710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001851","name":"Ministry of Earth Sciences","doi-asserted-by":"publisher","award":["MoES\/PAMCIDOM\/181\/2023 (E\u201014616)"],"award-info":[{"award-number":["MoES\/PAMCIDOM\/181\/2023 (E\u201014616)"]}],"id":[{"id":"10.13039\/501100001851","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Translesion DNA synthesis (TLS) is a fundamental biological process that enables DNA replication through various lesions to ensure genome stability and to prevent cell death due to replication fork collapse. Rev1, a member of Y\u2010family DNA polymerase (Pol), functions in concert with a B\u2010family enzyme Pol\u03b6 in promoting TLS through various lesions. Interestingly, for such a function, the catalytic activity of Rev1 seems to be dispensable in <jats:styled-content style=\"fixed-case\"><jats:italic>Saccharomyces cerevisiae<\/jats:italic><\/jats:styled-content>. Unlike Pol\u03b6, which possesses robust DNA polymerase activity, biochemical assays suggest that Rev1 predominantly incorporates a \u201cC\u201d opposite any templating residues, but the biological relevance of this activity of Rev1 remains elusive. Here we characterized Rev1 from <jats:italic><jats:styled-content style=\"fixed-case\">Candida albicans<\/jats:styled-content>,<\/jats:italic> an opportunistic fungal pathogen responsible for maximum casualties due to systemic candidiasis in immunosuppressed individuals. Concerted genetic analyses of several Rev1 mutants in various DNA\u2010damaging conditions suggested that in most lesion bypasses except 4\u2010NQO\u2010induced DNA lesions, the catalytic role of Rev1 is not important. However, simultaneous interactions of BRCT and the C\u2010terminal domain of Rev1 with PCNA and Pol\u03b6, respectively, enable Rev1 to be essential during TLS. DNA damage recovery and mutagenesis assays further confirmed the lesion\u2010specific roles of various domains of Rev1. Contrary to ex\u00a0vivo data, animal studies suggested that CaRev1 is dispensable for systemic candidiasis development. We discuss the possible involvement of other TLS DNA polymerases in DNA damage response while <jats:styled-content style=\"fixed-case\"><jats:italic>C. albicans<\/jats:italic><\/jats:styled-content> replicates and establishes itself in the host.<\/jats:p>","DOI":"10.1111\/mmi.70013","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T09:03:47Z","timestamp":1752743027000},"page":"342-357","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enzymatic and Structural Roles of <scp><i>Candida albicans<\/i><\/scp> Rev1 in <scp>DNA<\/scp> Damage Response and Disseminated Candidiasis"],"prefix":"10.1111","volume":"124","author":[{"given":"Satya Ranjan","family":"Sahu","sequence":"first","affiliation":[{"name":"Laboratory of Genomic Instability and Diseases, Department of Infectious Disease Biology Institute of Life Sciences  Bhubaneswar India"},{"name":"Regional Center of Biotechnology  Faridabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sushree Subhashree","family":"Parida","sequence":"additional","affiliation":[{"name":"Laboratory of Genomic Instability and Diseases, Department of Infectious Disease Biology Institute of Life Sciences  Bhubaneswar India"},{"name":"Regional Center of Biotechnology  Faridabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhabasha Gyanadeep","family":"Utkalaja","sequence":"additional","affiliation":[{"name":"Laboratory of Genomic Instability and Diseases, Department of Infectious Disease Biology Institute of Life Sciences  Bhubaneswar India"},{"name":"Regional Center of Biotechnology  Faridabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shreenath","family":"Nayak","sequence":"additional","affiliation":[{"name":"Laboratory of Genomic Instability and Diseases, Department of Infectious Disease Biology Institute of Life Sciences  Bhubaneswar 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