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Certain\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    exhibit insecticidal activity and produce virulence factors with cytotoxic and antimicrobial properties, positioning them as promising candidates for biological control. This study evaluated seven\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    strains with insecticidal activity and identified key virulence factors involved. The strains demonstrated varying degrees of insecticidal activity, with\n                    <jats:italic>Pseudomonas protegens<\/jats:italic>\n                    strains CHA0 and 59C being the most lethal, causing over 75% mortality and triggering a systemic melanization response in the insects. Genomic analysis revealed 175 virulence\u2010related genes shared across all strains and 16 genes specific to the highly insecticidal ones, including genes for antimicrobial compounds and insect toxins. Mutational analysis confirmed the roles of hydrogen cyanide, 2,4\u2010diacetylphloroglucinol, pyoluteorin, Fit toxin, and two\u2010partner secretion systems in\n                    <jats:italic>P. protegens<\/jats:italic>\n                    CHA0 insecticidal activity. This strain also exhibited insecticidal effects on adult\n                    <jats:italic>T. solanivora<\/jats:italic>\n                    and delayed egg hatching and pupal emergence. In microcosm assays,\n                    <jats:italic>P. protegens<\/jats:italic>\n                    CHA0 reduced tuber damage caused by\n                    <jats:italic>T. solanivora<\/jats:italic>\n                    larvae by up to 38%. These results suggest that\n                    <jats:italic>P. protegens<\/jats:italic>\n                    CHA0 is a promising biocontrol agent, providing a sustainable alternative to chemical pesticides to control\n                    <jats:italic>T. solanivora<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/mmi.70041","type":"journal-article","created":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T04:21:11Z","timestamp":1767673271000},"page":"159-172","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Specific Virulence Factors of\n                    <i>Pseudomonas<\/i>\n                    Strains in the Biocontrol of the Potato Pest\n                    <i>Tecia solanivora<\/i>"],"prefix":"10.1111","volume":"125","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9982-2335","authenticated-orcid":false,"given":"Luisa","family":"Pantoja","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Facultad de Ciencias, Pontificia Universidad Javeriana\u2014Sede Bogot\u00e1  Bogot\u00e1 Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8156-8110","authenticated-orcid":false,"given":"Pilar","family":"Vesga","sequence":"additional","affiliation":[{"name":"Centro de Biotecnolog\u00eda y Gen\u00f3mica de Plantas, Universidad Polit\u00e9cnica de Madrid  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0238-5445","authenticated-orcid":false,"given":"Ziv","family":"Arbeli","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Facultad de Ciencias, Pontificia Universidad Javeriana\u2014Sede Bogot\u00e1  Bogot\u00e1 Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5026-0618","authenticated-orcid":false,"given":"Vivian","family":"Boyac\u00e1\u2010V\u00e1squez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Facultad de Ciencias, Universidad Antonio Nari\u00f1o\u2014Sede Bogot\u00e1  Bogot\u00e1 Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2257-3056","authenticated-orcid":false,"given":"Javier","family":"Vanegas","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Facultad de Ciencias, Universidad Antonio Nari\u00f1o\u2014Sede Bogot\u00e1  Bogot\u00e1 Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jen.12634"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10526\u2010008\u20109157\u2010x"},{"key":"e_1_2_11_4_1","first-page":"162","volume-title":"Patogenicidad de Nematodos Entomopat\u00f3Genos Del Genero Steinernema y Heterorhabditis Sobre Larvas de Tecia solanivora en Ecuador","author":"Argotti E.","year":"2010"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/aab.12688"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2020.05.025"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.25100\/socolen.v30i1.9530"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1086\/525282"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.25100\/socolen.v36i1.9115"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2020.06.009"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.2144\/000112517"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3059.1998.00233.x"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2016.5"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396\u2010018\u20100317\u20104"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00100"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396\u2010023\u201001451\u20108"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy13122927"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.3c00064"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102583.2004.00454.x"},{"key":"e_1_2_11_21_1","first-page":"1","volume-title":"Pseudomonas: Molecular Biology and Biotechnology","author":"Holloway B. 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