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Without this sorting compartment, how cargo sorting to various subcellular destinations occurs within\n                    <jats:italic>Giardia<\/jats:italic>\n                    remains an open question. While the distribution of various Golgi\u2010associated SNAREs and Rabs has been documented in this parasite, the TRAPP (TRAnsport Protein Particle) complex, a guanine nucleotide exchange factor for Golgi\u2010associated Rabs, remained uncharacterized. Herein, we report that\n                    <jats:italic>Giardia<\/jats:italic>\n                    expresses a minimal set of TRAPP complex components, GlBet3, GlBet5, GlTrs23, and GlTrs31. Some of these components can interact with GlRab1a, GlRab11, and the COPII coat protein, GlSec23. Coupled with the colocalization and coimmunoprecipitation of GlBet3 and GlBet5, we propose the existence of a functional TRAPP complex in\n                    <jats:italic>Giardia<\/jats:italic>\n                    with an architecture that is different from that of yeast. While some interactions within this complex may be analogous to those in yeast, we find evidence of some unique interactions as well. The TRAPP genes are upregulated during encystation, and two components are associated with encystation\u2010specific vesicles. Besides the endomembrane system, the presence of GlBet3 and GlBet5 at the plasma membrane, membrane wrapping ventral disc periphery, and the median body indicates that the TRAPP complex may support unique features and functions of\n                    <jats:italic>Giardia<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/mmi.70049","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T11:47:03Z","timestamp":1768391223000},"page":"216-232","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evolutionarily Diverged\n                    <scp>TRAPP<\/scp>\n                    Components in\n                    <scp>\n                      <i>Giardia lamblia<\/i>\n                    <\/scp>\n                    That Lacks a Classical Golgi"],"prefix":"10.1111","volume":"125","author":[{"given":"Avishikta","family":"Chatterjee","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Bose Institute  Kolkata West Bengal India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuladip","family":"Jana","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Bose Institute  Kolkata West Bengal India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandipan","family":"Ganguly","sequence":"additional","affiliation":[{"name":"Division of Parasitology National Institute for Research in Bacterial Infections  Kolkata West Bengal India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srimonti","family":"Sarkar","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Bose Institute  Kolkata West Bengal 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