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The Rop\u2010induced increase in NO resistance was significantly reduced in several small noncoding RNA (sRNA) gene\u2010deficient EHEC mutants. Among these sRNAs, DsrA, ArcZ, and RprA were directly involved in the translational regulation of\n                    <jats:italic>rpoS<\/jats:italic>\n                    expression, suggesting that Rop modulates\n                    <jats:italic>rpoS<\/jats:italic>\n                    expression through sRNAs. To examine this mechanism, we generated sRNA gene\u2010deficient mutants with an additional deletion of the 5\u2032 untranslated region (5\u2032 UTR) of\n                    <jats:italic>rpoS<\/jats:italic>\n                    , which is required for translational regulation. The increase in NO resistance by Rop was restored in the double mutant, suggesting that this phenotype is mediated by Rop\u2010dependent interactions between sRNAs and the 5\u2032 UTR of\n                    <jats:italic>rpoS<\/jats:italic>\n                    mRNA. Furthermore, Rop promoted\n                    <jats:italic>rpoS<\/jats:italic>\n                    mRNA degradation, an effect that likely suppresses RpoS production and may thereby enhance NO resistance. Finally, an\n                    <jats:italic>hfq<\/jats:italic>\n                    \u2010deficient EHEC mutant exhibited no increase in NO resistance in the presence of Rop, indicating that Hfq is essential for Rop\u2010mediated NO resistance.\n                  <\/jats:p>","DOI":"10.1111\/mmi.70051","type":"journal-article","created":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T04:45:07Z","timestamp":1768970707000},"page":"250-267","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Rop\u2010Mediated Suppression of\n                    <scp>RpoS<\/scp>\n                    Production Increases Resistance to Nitric Oxide"],"prefix":"10.1111","volume":"125","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8865-1884","authenticated-orcid":false,"given":"Takeshi","family":"Shimizu","sequence":"first","affiliation":[{"name":"Department of Molecular Infectiology Graduate School of Medicine, Chiba University  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Molecular Infectiology Graduate School of Medicine, Chiba University  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiyou","family":"Fukumoto","sequence":"additional","affiliation":[{"name":"Department of Molecular Infectiology Graduate School of Medicine, Chiba University  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Hamabata","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease National Institute of Global Health and Medicine, Japan Institute for Health Security  Shinjuku\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,20]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-090110-102946"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf103"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.3.656-662.1997"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-092611-150135"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.20.6313"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.20.4688"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1903461116"},{"key":"e_1_2_12_9_1","first-page":"E8968","article-title":"Enterotoxigenic E. coli Virulence Gene Regulation in Human Infections","volume":"115","author":"Crofts A. 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