{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T11:15:55Z","timestamp":1788952555535,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP22K19138"],"award-info":[{"award-number":["JP22K19138"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP25K01938"],"award-info":[{"award-number":["JP25K01938"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJCR25J3"],"award-info":[{"award-number":["JPMJCR25J3"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Heterocyst\u2010specific polysaccharide (Hep) is an essential component of the heterocyst envelope in the filamentous cyanobacterium\n                    <jats:italic>Anabaena<\/jats:italic>\n                    sp. PCC 7120; however, the regulatory mechanisms that control Hep layer formation remain poorly understood. Herein, we reveal that Hep layer formation is regulated by a phosphorylation\u2010dependent partner\u2010switching\u2010like system involving the phosphatase HenR and the STAS domain\u2010containing glycosyltransferase All4160. A nonphosphorylatable All4160 variant restores Hep formation in a\n                    <jats:italic>henR<\/jats:italic>\n                    disruptant, indicating that phosphorylation negatively regulates All4160 function. Using a bacterial two\u2010hybrid assay, we identified two candidate kinases that interact with All4160, that is, Alr3423 and All2284, which phosphorylate All4160 in\u00a0vitro. Genetic analysis revealed that deletion of alr3423, not all2284, suppresses the defect of the\n                    <jats:italic>henR<\/jats:italic>\n                    disruptant, indicating that Alr3423 plays a key role in regulating All4160 in\u00a0vivo. This study identifies a phosphorylation switch that controls Hep layer formation and reveals a regulatory mechanism acting directly on a polysaccharide biosynthetic enzyme.\n                  <\/jats:p>","DOI":"10.1111\/mmi.70091","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T05:03:16Z","timestamp":1782190996000},"page":"237-243","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Phosphorylation\u2010Dependent Partner\u2010Switching\u2010Like Module Regulates a Glycosyltransferase Required for Heterocyst Polysaccharide Layer Formation in\n                    <i>Anabaena<\/i>\n                    sp. Strain\n                    <scp>PCC<\/scp>\n                    7120"],"prefix":"10.1111","volume":"126","author":[{"given":"Mai","family":"Harada","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Graduate School of Science Tokyo Metropolitan University  Hachioji Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9215-9948","authenticated-orcid":false,"given":"Satoshi","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering Saitama University  Saitama Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeki","family":"Ehira","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Graduate School of Science Tokyo Metropolitan University  Hachioji Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1461-0248.2002.00364.x"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01670.x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1758-2229.12620"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05049.x"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/10.3.97"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000549"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.6.1998-2005.1997"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01669-05"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2018.04.006"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.3.1114-1123.2005"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/8.5.205"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.104361"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a000315"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.66538"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26747-0"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14061"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00343-07"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.10.18.2265"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.16.4233-4242.1998"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.70091","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mmi.70091","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.70091","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.70091","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T10:41:10Z","timestamp":1788950470000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.70091"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,22]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/mmi.70091"],"URL":"https:\/\/doi.org\/10.1111\/mmi.70091","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,22]]},"assertion":[{"value":"2026-05-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}