{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T11:15:05Z","timestamp":1788952505657,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI15650"],"award-info":[{"award-number":["AI15650"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001793","name":"Queensland University of Technology","doi-asserted-by":"publisher","award":["2017HIG0119"],"award-info":[{"award-number":["2017HIG0119"]}],"id":[{"id":"10.13039\/501100001793","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001792","name":"James Cook University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001792","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>fabH<\/jats:italic>\n                    gene encodes the 3\u2010ketoacyl\u2010ACP synthase III that initiates fatty acid synthesis. Deletion of the\n                    <jats:italic>fabH<\/jats:italic>\n                    gene results in severely limited fatty acid synthesis and tiny cells that are unusually sensitive to antibiotics. Genetic investigations identified the\n                    <jats:italic>yiiD<\/jats:italic>\n                    gene, now called\n                    <jats:italic>madA<\/jats:italic>\n                    , that encodes a malonyl\u2010ACP decarboxylase that suppresses the\n                    <jats:italic>\u2206fabH<\/jats:italic>\n                    phenotype, but only when\n                    <jats:italic>madA<\/jats:italic>\n                    is multicopy. We selected vancomycin\u2010resistant suppressor derivatives of a\n                    <jats:italic>\u2206fabH<\/jats:italic>\n                    strain. Although such chromosomal mutations were generally rare and weak, suppressor strain s1 (a single C\u2010T transition) restored both wild\u2010type growth, high\u2010level vancomycin resistance, and wild\u2010type fatty acid synthesis by creation of a new promoter within the coding sequence of a gene upstream of\n                    <jats:italic>madA<\/jats:italic>\n                    . This provides a caveat to the extensive effort to develop FabH inhibitors as antibacterial drugs.\n                  <\/jats:p>","DOI":"10.1111\/mmi.70092","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T14:32:56Z","timestamp":1782484376000},"page":"244-251","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Single\u2010Nucleotide Substitution Generates a\n                    <i>de Novo<\/i>\n                    Promoter That Activates a Latent Metabolic Bypass in\n                    <i>Escherichia coli<\/i>"],"prefix":"10.1111","volume":"126","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8252-2616","authenticated-orcid":false,"given":"Qiaoqiao","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Microbiology, School of Molecular and Cellular Biology University of Illinois  Urbana Illinois USA"},{"name":"State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology Chinese Academy of Sciences  Guangzhou Guangdong China"},{"name":"Laboratory of Tropical Marine Bio\u2010Resources and Ecology, South China Sea Institute of Oceanology Chinese Academy of Sciences  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Molecular and Cellular Biology University of Illinois  Urbana Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jilong","family":"Qin","sequence":"additional","affiliation":[{"name":"Centre for Immunology and Infection Control, School of Biomedical Sciences Queensland University of Technology  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makrina","family":"Totsika","sequence":"additional","affiliation":[{"name":"Centre for Immunology and Infection Control, School of Biomedical 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