{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:03:21Z","timestamp":1791378201689,"version":"4.3.2"},"reference-count":54,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32402918"],"award-info":[{"award-number":["32402918"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32273033"],"award-info":[{"award-number":["32273033"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Toxoplasmosis caused by the Apicomplexan parasite\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    is a significant health threat to immunocompromised individuals and newborns. This parasite has a complex life cycle that is well controlled to achieve optimal transmission and pathogenesis. Acute phase of the disease is caused by rapid proliferation of tachyzoites, which has a highly coordinated and tightly regulated cell cycle to allow parasite propagation. Tachyzoite cell cycle has five partially overlapping phases (G1, S, G2, M and C) with distinct gene expression patterns and cellular activities, yet the underlying regulatory mechanisms are not well understood. In this study, we show that the AP2 family transcription factor AP2XI\u20103 has important roles in regulating the cell cycle to progress through the G1 phase. Depletion of AP2XI\u20103 resulted in cell cycle retention at G1, leading to growth inhibition of tachyzoites. RNA\u2010Seq and CUT&amp;Tag analyses revealed that AP2XI\u20103 regulates the transcription of genes involved in nucleic acid metabolism, RNA biogenesis and processing, which are consistent with the cellular activities of G1 phase in preparing biomass for cell cycle progression. Moreover, AP2XI\u20103 binds to the conserved DNA motif, TRP\u20102, a sequence widely distributed in the promoters of genes that exhibit peak expression during the G1 phase. Together, these findings underscore the essential role of AP2XI\u20103 in the tight regulation of the G1 phase, highlighting its potential as a therapeutic target for drug development.\n                  <\/jats:p>","DOI":"10.1111\/mmi.70099","type":"journal-article","created":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T14:41:33Z","timestamp":1784990493000},"page":"329-342","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>AP2XI<\/scp>\n                    \u20103 is Critical for the Cell Cycle of\n                    <i>Toxoplasma<\/i>\n                    to Progress Through the\n                    <scp>G1<\/scp>\n                    Phase"],"prefix":"10.1111","volume":"126","author":[{"given":"Fuqiang","family":"Fan","sequence":"first","affiliation":[{"name":"Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi\u2010Omics of MARA Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences  Shenzhen China"},{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine Huazhong Agricultural University  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Yin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine Huazhong Agricultural University  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anqi","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine Huazhong Agricultural University  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilan","family":"Xue","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine Huazhong Agricultural University  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinling","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine Huazhong Agricultural University  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangfan","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine Huazhong Agricultural University  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0103-271X","authenticated-orcid":false,"given":"Bang","family":"Shen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine Huazhong Agricultural University  Wuhan Hubei China"},{"name":"Hubei Hongshan Laboratory  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006483"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394309-5.00001-8"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2010.01514.x"},{"key":"e_1_2_12_5_1","first-page":"2834","article-title":"STREME: Accurate and Versatile Sequence Motif Discovery","volume":"37","author":"Bailey T. 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