{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T06:05:08Z","timestamp":1790057108192,"version":"4.0.1"},"reference-count":111,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cyclic di\u2010GMP is ubiquitous in Bacteria, including members of the deepest branching phyla, but has not yet been detected in Archaea. Thus, whether cyclic di\u2010GMP was present as a signaling nucleotide in the last universal common ancestor (LUCA) of Bacteria and Archaea remains unknown. In this work, bioinformatic analyses and structural modelling identified GGDEF domain proteins in archaeal isolates and encoded by metagenomes of confirmed archaeal origin. In particular, in bacterial model organisms, phenotypic and in\u00a0vivo assays, in combination with catalytic mutants, suggest that selected archaeal GGDEF domain proteins possess diguanylate cyclase activity. These include the complex REC\n                    <jats:sub>S<\/jats:sub>\n                    \u2010PAS\/PAC\u2010PocR\u2010GGDEF\u2010HD\u2010GYP domain protein of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Methanocella arvoryzae<\/jats:italic>\n                    <\/jats:styled-content>\n                    MRE50, a member of the\n                    <jats:italic>Stenosarchaea<\/jats:italic>\n                    order\n                    <jats:italic>Methanocellales<\/jats:italic>\n                    . While cyclic di\u2010GMP signaling proteins are ubiquitous in Bacteria, their presence seems to be more sporadic in Archaea. It is currently unclear whether cyclic di\u2010GMP signaling proteins have been lost in some lineages, secondarily introduced by horizontal gene transfer into others, or whether both scenarios have occurred.\n                  <\/jats:p>","DOI":"10.1111\/mmi.70117","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:37:00Z","timestamp":1790055420000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Archaeal Genes Code for\n                    <scp>GGDEF<\/scp>\n                    Domain Proteins With Diguanylate Cyclase Activity"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9707-5302","authenticated-orcid":false,"given":"Roman","family":"Sidorov","sequence":"first","affiliation":[{"name":"Department of Microbiology, Tumor and Cell Biology, Biomedicum Karolinska Instittuet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7885-8108","authenticated-orcid":false,"given":"Li","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Tumor and Cell Biology, Biomedicum Karolinska Instittuet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0938-5125","authenticated-orcid":false,"given":"Ali","family":"Dadvar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Tumor and Cell Biology, Biomedicum Karolinska Instittuet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2648-1952","authenticated-orcid":false,"given":"Irfan","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Tumor and Cell Biology, Biomedicum Karolinska Instittuet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6247-0938","authenticated-orcid":false,"given":"Stefan","family":"Spring","sequence":"additional","affiliation":[{"name":"Leibniz Institute DSMZ\u2010German Collection of Microorganisms and Cell Cultures Braunschweig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3909-7201","authenticated-orcid":false,"given":"J\u00f6rg","family":"Overmann","sequence":"additional","affiliation":[{"name":"Leibniz Institute DSMZ\u2010German Collection of Microorganisms and Cell Cultures Braunschweig Germany"},{"name":"Braunschweig University of Technology, Institute for Microbiology Braunschweig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3812-6621","authenticated-orcid":false,"given":"Ute","family":"R\u00f6mling","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Tumor and Cell Biology, Biomedicum Karolinska Instittuet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1111\/mmi.12428","article-title":"The EAL\u2010Like Protein STM1697 Regulates Virulence Phenotypes, Motility and Biofilm Formation in \n                     Salmonella typhimurium","volume":"90","author":"Ahmad I.","year":"2013","journal-title":"Molecular Microbiology"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic Local Alignment Search Tool","volume":"215","author":"Altschul S. 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