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Its homologue\n                    <jats:styled-content style=\"fixed-case\">Sac6p<\/jats:styled-content>\n                    has been shown to play a critical role in endocytosis and diverse cellular processes in\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>\n                      accharomyces cerevisiae.\n                    <\/jats:italic>\n                    \u2005\n                    <jats:styled-content style=\"fixed-case\">FgFim<\/jats:styled-content>\n                    from the wheat scab pathogenic fungus\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>\n                      usarium graminearum\n                    <\/jats:italic>\n                    strain\n                    <jats:styled-content style=\"fixed-case\">Y2021A<\/jats:styled-content>\n                    , which is highly resistant to the fungicide\n                    <jats:styled-content style=\"fixed-case\">JS399<\/jats:styled-content>\n                    \u201019, was identified by screening a mutant library generated by\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">HPH\u2010HSV<\/jats:styled-content>\n                      \u2010tk\n                    <\/jats:italic>\n                    cassette\u2010mediated integration. The functions of\n                    <jats:styled-content style=\"fixed-case\">FgFim<\/jats:styled-content>\n                    were evaluated by constructing a deletion mutant of\n                    <jats:styled-content style=\"fixed-case\">FgFim<\/jats:styled-content>\n                    , designated \u0394\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>FgFim<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>\u201015<\/jats:italic>\n                    . The deletion mutant exhibited a reduced rate of mycelial growth, reduced conidiation, delayed conidium germination, irregularly shaped hyphae, a lack of sexual reproduction on autoclaved wheat kernels and a dramatic decrease in resistance to\n                    <jats:styled-content style=\"fixed-case\">JS<\/jats:styled-content>\n                    399\u201019. \u0394\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>FgFim<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>\u201015<\/jats:italic>\n                    also exhibited increased sensitivity to diverse metal cations, to agents that induce osmotic stress and oxidative stress, and to agents that damage the cell membrane and cell wall. Pathogenicity assays showed that the virulence of the\n                    <jats:styled-content style=\"fixed-case\">FgFim<\/jats:styled-content>\n                    deletion mutant on flowering wheat heads was impaired, which was consistent with its reduced production of the toxin deoxynivalenol in host tissue. All of these defects were restored by genetic complementation of the mutant with the parental\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">FgFim<\/jats:styled-content>\n                    <\/jats:italic>\n                    gene. Quantitative real\u2010time polymerase chain reaction (\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    ) assays showed that the basal expression of three\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">Cyp51<\/jats:styled-content>\n                    <\/jats:italic>\n                    genes, which encode sterol 14\u03b1\u2010demethylase, was significantly lower in the mutant than in the parental strain. The results of this study indicate that\n                    <jats:styled-content style=\"fixed-case\">FgFim<\/jats:styled-content>\n                    plays a critical role in the regulation of resistance to\n                    <jats:styled-content style=\"fixed-case\">JS399<\/jats:styled-content>\n                    \u201019 and in various cellular processes in\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>\n                      .\u2009graminearum.\n                    <\/jats:italic>\n                  <\/jats:p>","DOI":"10.1111\/mpp.12108","type":"journal-article","created":{"date-parts":[[2013,12,3]],"date-time":"2013-12-03T06:57:45Z","timestamp":1386053865000},"page":"488-499","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["<scp>FgFim<\/scp>\n                    , a key protein regulating resistance to the fungicide\n           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