{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T02:39:44Z","timestamp":1781923184990,"version":"3.54.5"},"reference-count":60,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,10,27]],"date-time":"2014-10-27T00:00:00Z","timestamp":1414368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Citrus Research and Development Foundation, Inc. (CRDF)","award":["572"],"award-info":[{"award-number":["572"]}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2015,6]]},"abstract":"Summary<\/jats:title>\n \n The bacterial agent of citrus canker disease (\n \n X<\/jats:styled-content>\n anthomonas citri\n <\/jats:italic>\n ssp.\n citri<\/jats:italic>\n ,\n \n X<\/jats:styled-content>\n cc\n <\/jats:italic>\n ) has caused tremendous economic losses to the citrus industry around the world. Pathogen\u2010associated molecular pattern (\n PAMP<\/jats:styled-content>\n )\u2010triggered immunity (\n PTI<\/jats:styled-content>\n ) is important to plant immunity. In this study, we compared the defence responses of citrus canker\u2010resistant and citrus canker\u2010susceptible genotypes to the\n \n X<\/jats:styled-content>\n cc\n <\/jats:italic>\n \u2010derived\n PAMP<\/jats:styled-content>\n flg22 (\n Xflg22<\/jats:styled-content>\n ) by analysing the expression of 20 citrus defence\u2010associated genes. We showed that, in the most resistant genotype, \u2018Nagami\u2019 kumquat, there was significant induction of several defence genes (\n \n EDS1<\/jats:styled-content>\n <\/jats:italic>\n ,\n \n NDR1<\/jats:styled-content>\n <\/jats:italic>\n ,\n \n PBS1<\/jats:styled-content>\n <\/jats:italic>\n ,\n \n RAR1<\/jats:styled-content>\n <\/jats:italic>\n ,\n \n SGT1<\/jats:styled-content>\n <\/jats:italic>\n ,\n \n PAL1<\/jats:styled-content>\n <\/jats:italic>\n ,\n \n NPR2<\/jats:styled-content>\n <\/jats:italic>\n and\n \n NPR3<\/jats:styled-content>\n <\/jats:italic>\n ) as early as 6\u2009h and up to 72\u2009h after\n X<\/jats:styled-content>\n flg22 treatment. At the other end of the spectrum, highly susceptible \u2018Duncan\u2019 grapefruit showed no induction of the same defence genes, even 120\u2009h after treatment. Citrus genotypes with partial levels of resistance showed intermediate levels of transcriptional reprogramming that correlated with their resistance level.\n X<\/jats:styled-content>\n flg22 also triggered a rapid oxidative burst in all genotypes which was higher and accompanied by the induction of\n PTI<\/jats:styled-content>\n marker genes (\n \n WRKY22<\/jats:styled-content>\n <\/jats:italic>\n and\n \n GST1<\/jats:styled-content>\n <\/jats:italic>\n ) only in the more resistant genotypes. Pretreatment with\n X<\/jats:styled-content>\n flg22 prior to\n \n X<\/jats:styled-content>\n cc\n <\/jats:italic>\n inoculation inhibited bacterial growth in kumquat, but not in grapefruit. A flagellin\u2010deficient\n \n X<\/jats:styled-content>\n cc\n <\/jats:italic>\n strain (\n Xcc\u0394fliC<\/jats:italic>\n ) showed greater growth increase relative to wild\u2010type\n \n X<\/jats:styled-content>\n cc\n <\/jats:italic>\n in kumquat than in grapefruit. Taken together, our results indicate that\n X<\/jats:styled-content>\n flg22 initiates strong\n PTI<\/jats:styled-content>\n in canker\u2010resistant genotypes, but not in susceptible ones, and that a robust induction of\n PTI<\/jats:styled-content>\n is an important component of citrus resistance to canker.\n <\/jats:p>","DOI":"10.1111\/mpp.12206","type":"journal-article","created":{"date-parts":[[2014,9,18]],"date-time":"2014-09-18T04:27:50Z","timestamp":1411014470000},"page":"507-520","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Responsiveness of different citrus genotypes to the\n \n X<\/scp>\n anthomonas citri\n <\/i>\n ssp.\n citri<\/i>\n \u2010derived pathogen\u2010associated molecular pattern (\n PAMP<\/scp>\n ) flg22 correlates with resistance to citrus canker"],"prefix":"10.1111","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0333-9451","authenticated-orcid":false,"given":"Qingchun","family":"Shi","sequence":"first","affiliation":[{"name":"Horticultural Sciences Department University of Florida P.O. Box 110690 Gainesville FL 32611 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicente J.","family":"Febres","sequence":"additional","affiliation":[{"name":"Horticultural Sciences Department University of Florida P.O. Box 110690 Gainesville FL 32611 USA"},{"name":"Plant Molecular and Cellular Biology Program University of Florida P.O. Box 110690 Gainesville FL 32611 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey B.","family":"Jones","sequence":"additional","affiliation":[{"name":"Plant Molecular and Cellular Biology Program University of Florida P.O. Box 110690 Gainesville FL 32611 USA"},{"name":"Plant Pathology Department University of Florida P.O. Box 110680 Gainesville FL 32611 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria A.","family":"Moore","sequence":"additional","affiliation":[{"name":"Horticultural Sciences Department University of Florida P.O. Box 110690 Gainesville FL 32611 USA"},{"name":"Plant Molecular and Cellular Biology Program University of Florida P.O. Box 110690 Gainesville FL 32611 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,10,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.17.10306"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.15.7327"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/415977a"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1067554"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.16.7608"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.57.032905.105346"},{"key":"e_1_2_6_8_1","volume-title":"Comprehensive Report on Citrus Canker in Florida","author":"Bronson C.H.","year":"2007"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.2307\/3869945"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.14.6597"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/35081161"},{"key":"e_1_2_6_12_1","first-page":"6","volume-title":"2011 Florida Citrus Pest Management Guide: Citrus Canker","author":"Dewdney M.M.","year":"2011"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)62404-3"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1999.00265.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-02-10-0047"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.10.6.947"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-22-1-0086"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11162"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80265-8"},{"key":"e_1_2_6_20_1","volume-title":"Citrus Canker","author":"Goto M.","year":"1992"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1094\/PD-77-1004"},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","unstructured":"Gottwald T.R. 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