{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:37:24Z","timestamp":1790836644229,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,5,12]],"date-time":"2015-05-12T00:00:00Z","timestamp":1431388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Key Basic Research Programme","award":["2012CB114000"],"award-info":[{"award-number":["2012CB114000"]}]},{"name":"National Science and Technology Support Programme","award":["2012BAD19B01"],"award-info":[{"award-number":["2012BAD19B01"]}]},{"DOI":"10.13039\/501100010042","name":"Special Fund for Agro-scientific Research in the Public Interest","doi-asserted-by":"crossref","award":["201303023"],"award-info":[{"award-number":["201303023"]}],"id":[{"id":"10.13039\/501100010042","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2016,1]]},"abstract":"Summary<\/jats:title>\n \n \n F<\/jats:styled-content>\n usarium graminearum\n <\/jats:italic>\n , the causal agent of\n F<\/jats:styled-content>\n usarium head blight, is a common pathogen on small grain cereals worldwide and produces various trichothecenes [deoxynivalenol (\n DON<\/jats:styled-content>\n ) is predominant] during infection. A previous study has revealed that\n DON<\/jats:styled-content>\n production is positively correlated with the occurrence of carbendazim (\n MBC<\/jats:styled-content>\n ) resistance. Here, we identified and characterized two putative genes encoding hexokinase in\n \n F<\/jats:styled-content>\n .\u2009graminearum\n <\/jats:italic>\n (\n \n FgHXK1<\/jats:styled-content>\n <\/jats:italic>\n and\n \n FgHXK2<\/jats:styled-content>\n <\/jats:italic>\n ), which is a rate\u2010limiting enzyme in\n DON<\/jats:styled-content>\n biosynthesis. The expression level of hexokinase genes and the production of pyruvate, which is the precursor of\n DON<\/jats:styled-content>\n , were up\u2010regulated in the\n MBC<\/jats:styled-content>\n \u2010resistant strain, indicating that hexokinase genes might be involved in increased\n DON<\/jats:styled-content>\n production. Phylogenetic and comparative analyses indicated that\n \n FgHXK1<\/jats:styled-content>\n <\/jats:italic>\n was the predominant hexokinase gene. Gene disruption showed that \u0394\n \n FgHXK1<\/jats:styled-content>\n <\/jats:italic>\n severely affected\n DON<\/jats:styled-content>\n production, indicating that\n \n FgHXK1<\/jats:styled-content>\n <\/jats:italic>\n played a role in the regulation of\n DON<\/jats:styled-content>\n biosynthesis. Morphological characterization showed that \u0394\n \n FgHXK1<\/jats:styled-content>\n <\/jats:italic>\n led to inhibited vegetative growth and conidiation. Sensitivity tests to\n MBC<\/jats:styled-content>\n and various stresses indicated that both \u0394\n \n FgHXK1<\/jats:styled-content>\n <\/jats:italic>\n and \u0394\n \n FgHXK2<\/jats:styled-content>\n <\/jats:italic>\n mutants showed no significant difference from parental strains. Pathogencity assays showed that \u0394\n \n FgHXK1<\/jats:styled-content>\n <\/jats:italic>\n mutants lost virulence on wheat head and corn stigma; however, they showed no change in sexual reproduction. The\n \n FgHXK1<\/jats:styled-content>\n \u2010\n <\/jats:italic>\n overexpressing transformants were obtained subsequently. Their pyruvate and\n DON<\/jats:styled-content>\n production was confirmed to be increased, indicating that\n \n FgHXK1<\/jats:styled-content>\n <\/jats:italic>\n positively regulated\n DON<\/jats:styled-content>\n biosynthesis. Although additional defects appeared in overexpression mutants,\n MBC<\/jats:styled-content>\n sensitivity showed no change. All of the results indicated that the transcriptional level of\n \n FgHXK1<\/jats:styled-content>\n <\/jats:italic>\n regulated\n DON<\/jats:styled-content>\n biosynthesis, but showed no direct relationship with\n MBC<\/jats:styled-content>\n resistance.\n <\/jats:p>","DOI":"10.1111\/mpp.12258","type":"journal-article","created":{"date-parts":[[2015,3,24]],"date-time":"2015-03-24T14:06:19Z","timestamp":1427205979000},"page":"16-28","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Hexokinase plays a critical role in deoxynivalenol (\n DON<\/scp>\n ) production and fungal development in\n Fusarium graminearum<\/i>"],"prefix":"10.1111","volume":"17","author":[{"given":"Leigang","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University Nanjing 210095 China"},{"name":"State & Local Joint Engineering Research Center of Green Pesticide Invention and Application Nanjing Agricultural University Nanjing 210095 China"},{"name":"Institute of Agricultural Products Processing Jiangsu Academy of Agricultural Sciences Nanjing 210014 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baicun","family":"Li","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University Nanjing 210095 China"},{"name":"State & Local Joint Engineering Research Center of Green Pesticide Invention and Application Nanjing Agricultural University Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University Nanjing 210095 China"},{"name":"State & Local Joint Engineering Research Center of Green Pesticide Invention and Application Nanjing Agricultural University Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Jia","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University Nanjing 210095 China"},{"name":"State & Local Joint Engineering Research Center of Green Pesticide Invention and Application Nanjing Agricultural University Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingguo","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University Nanjing 210095 China"},{"name":"State & Local Joint Engineering Research Center of Green Pesticide Invention and Application Nanjing Agricultural University Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/15569540903092142"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-112"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.3.497-516.2003"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2003.12.002"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2008.03.014"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.1449"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.724"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5483.1337"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-9-0775"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00362-09"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2004.00252.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.151130"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025311"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.052506-0"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.70183"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbn017"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(77)90544-6"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.6.11.4046"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2006.00351.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/20.22.6115"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1080585"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3059.1995.tb02773.x"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0377-8401(98)00278-8"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.61.5.1923-1930.1995"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.2050"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/02652030500058403"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3550625"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/006338-0"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.70.1.253-282.2006"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2007.03.001"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.2307\/3761794"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO.2000.90.1.17"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1385\/CT:5:3:293"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmpp.2013.07.005"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-22-9-1143"},{"key":"e_1_2_6_38_1","first-page":"365","article-title":"Study 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