{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T15:33:45Z","timestamp":1790436825323,"version":"4.1.0"},"reference-count":64,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,3,29]],"date-time":"2016-03-29T00:00:00Z","timestamp":1459209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    HRT is a plant coiled\u2010coil, nucleotide\u2010binding and leucine\u2010rich repeat (CC\u2010NB\u2010LRR) disease resistance protein that triggers the hypersensitive response (HR) on recognition of\n                    <jats:italic>Turnip crinkle virus<\/jats:italic>\n                    (TCV) coat protein (CP). The molecular mechanism and significance of HR\u2010mediated cell death for TCV resistance have not been fully elucidated. To identify the genes involved in HRT\/TCV CP\u2010mediated HR in\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    , we performed virus\u2010induced gene silencing (VIGS) of 459 expressed sequence tags (ESTs) of pathogen\u2010responsive\n                    <jats:italic>Capsicum annuum<\/jats:italic>\n                    genes. VIGS of\n                    <jats:italic>CaBLP5<\/jats:italic>\n                    , which encodes an endoplasmic reticulum (ER)\u2010associated immunoglobulin\u2010binding protein (BiP), silenced\n                    <jats:italic>NbBiP4<\/jats:italic>\n                    and\n                    <jats:italic>NbBiP5<\/jats:italic>\n                    and significantly reduced HRT\u2010mediated HR. The induction of ER stress\u2010responsive genes and the accumulation of ER\u2010targeted BiPs in response to HRT\u2010mediated HR suggest that ER is involved in HR in\n                    <jats:italic>N. benthamiana<\/jats:italic>\n                    .\n                    <jats:italic>BiP4\/5<\/jats:italic>\n                    silencing significantly down\u2010regulated\n                    <jats:italic>HRT<\/jats:italic>\n                    at the mRNA and protein levels, and affected\n                    <jats:italic>SGT1<\/jats:italic>\n                    and\n                    <jats:italic>HSP90<\/jats:italic>\n                    expression. Co\u2010expression of\n                    <jats:italic>TCV CP<\/jats:italic>\n                    in\n                    <jats:italic>BiP4\/5<\/jats:italic>\n                    \u2010silenced plants completely abolished\n                    <jats:italic>HRT<\/jats:italic>\n                    induction. Transient expression of\n                    <jats:italic>TCV CP<\/jats:italic>\n                    alone induced selected ER stress\u2010responsive gene transcripts only in\n                    <jats:italic>Tobacco rattle virus<\/jats:italic>\n                    (TRV)\u2010infected plants, and most of these genes were induced by HRT\/TCV CP, except for\n                    <jats:italic>bZIP60<\/jats:italic>\n                    , which was induced specifically in response to HRT\/TCV CP. TCV CP\u2010mediated induction of ER stress\u2010responsive genes still occurred in\n                    <jats:italic>BiP4\/5<\/jats:italic>\n                    \u2010silenced plants, but HRT\/TCV CP\u2010mediated induction of these genes was defective. Tunicamycin, a chemical that inhibits protein\n                    <jats:italic>N<\/jats:italic>\n                    \u2010glycosylation, inhibited HRT\u2010mediated HR, suggesting that ER has a role in HR regulation. These results indicate that BiP and ER, which modulate pattern recognition receptors in innate immunity, also regulate R protein\u2010mediated resistance.\n                  <\/jats:p>","DOI":"10.1111\/mpp.12369","type":"journal-article","created":{"date-parts":[[2016,1,18]],"date-time":"2016-01-18T18:40:42Z","timestamp":1453142442000},"page":"1382-1397","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Endoplasmic reticulum stress responses function in the HRT\u2010mediated hypersensitive response in\n                    <i>Nicotiana benthamiana<\/i>"],"prefix":"10.1111","volume":"17","author":[{"given":"Ju Yeon","family":"Moon","sequence":"first","affiliation":[{"name":"Molecular Biofarming Research Center KRIBB Daejeon 305\u2010600 South Korea"},{"name":"Department of Biosystems and Bioengineering UST Daejeon 305\u2010350 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong Hee","family":"Lee","sequence":"additional","affiliation":[{"name":"Molecular Biofarming Research Center KRIBB Daejeon 305\u2010600 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang\u2010Sik","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Horticulture Kyung Hee University Yongin 446\u2010701 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Gu","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Biology Texas State University San Marcos TX 78666 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong Mee","family":"Park","sequence":"additional","affiliation":[{"name":"Molecular Biofarming Research Center KRIBB Daejeon 305\u2010600 South Korea"},{"name":"Department of Biosystems and Bioengineering UST Daejeon 305\u2010350 South 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