{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:40:56Z","timestamp":1791222056214,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,5,8]],"date-time":"2016-05-08T00:00:00Z","timestamp":1462665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Spanish grant agency DGICYT","award":["BIO2014-54862-R"],"award-info":[{"award-number":["BIO2014-54862-R"]}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    During virus infection, specific viral component\u2013host factor interaction elicits the transcriptional reprogramming of diverse cellular pathways.\n                    <jats:italic>Alfalfa mosaic virus<\/jats:italic>\n                    (AMV) can establish a compatible interaction in tobacco and Arabidopsis hosts. We show that the coat protein (CP) of AMV interacts directly with transcription factor (TF) ILR3 of both species. ILR3 is a basic helix\u2013loop\u2013helix (bHLH) family member of TFs, previously proposed to participate in diverse metabolic pathways. ILR3 has been shown to regulate NEET in Arabidopsis, a critical protein in plant development, senescence, iron metabolism and reactive oxygen species (ROS) homeostasis. We show that the AMV CP\u2013ILR3 interaction causes a fraction of this TF to relocate from the nucleus to the nucleolus. ROS, pathogenesis\u2010related protein 1 (PR1) mRNAs, salicylic acid (SA) and jasmonic acid (JA) contents are increased in healthy Arabidopsis loss\u2010of\u2010function ILR3 mutant (ilr3.2) plants, which implicates ILR3 in the regulation of plant defence responses. In AMV\u2010infected wild\u2010type (wt) plants, NEET expression is reduced slightly, but is induced significantly in\n                    <jats:italic>ilr3.2<\/jats:italic>\n                    mutant plants. Furthermore, the accumulation of SA and JA is induced in Arabidopsis wt\u2010infected plants. AMV infection in\n                    <jats:italic>ilr3.2<\/jats:italic>\n                    plants increases JA by over 10\u2010fold, and SA is reduced significantly, indicating an antagonist crosstalk effect. The accumulation levels of viral RNAs are decreased significantly in\n                    <jats:italic>ilr3.2<\/jats:italic>\n                    mutants, but the virus can still systemically invade the plant. The AMV CP\u2013ILR3 interaction may down\u2010regulate a host factor, NEET, leading to the activation of plant hormone responses to obtain a hormonal equilibrium state, where infection remains at a level that does not affect plant viability\n                  <\/jats:p>","DOI":"10.1111\/mpp.12388","type":"journal-article","created":{"date-parts":[[2016,2,29]],"date-time":"2016-02-29T11:45:38Z","timestamp":1456746338000},"page":"173-186","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["The coat protein of\n                    <i>Alfalfa mosaic virus<\/i>\n                    interacts and interferes with the transcriptional activity of the bHLH transcription factor ILR3 promoting salicylic acid\u2010dependent defence signalling response"],"prefix":"10.1111","volume":"18","author":[{"given":"Frederic","family":"Aparicio","sequence":"first","affiliation":[{"name":"Department of Molecular and Evolutionary Plant Virology Instituto de Biolog\u00eda Molecular y Celular de Plantas (IBMCP) (UPV\u2010CSIC) Ingeniero Fausto Elio s\/n 46022 Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicente","family":"Pall\u00e1s","sequence":"additional","affiliation":[{"name":"Department of Molecular and Evolutionary Plant Virology Instituto de Biolog\u00eda Molecular y Celular de Plantas (IBMCP) (UPV\u2010CSIC) Ingeniero Fausto Elio s\/n 46022 Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1332"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12204"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0042-6822(03)00284-8"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.104.058958"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81696-0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-02-14-0035-R"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.207860"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.43.101804.120505"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.39.1.419"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2015.11.003"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.45.062806.094422"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2013.11.018"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.2003.01656.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2015.01.001"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msg088"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-04-12-0079-R"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.104.056028"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.180042"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-19-0350"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.107.055566"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1132563"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002467"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.110.074096"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.106476"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.113.111658"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1364-3703.2000.00002.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.097634"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2007.03212.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.19.3059"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2013.07.023"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2004.12.010"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707189104"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.034603-0"},{"key":"e_1_2_6_35_1","first-page":"461","article-title":"Detection of plant RNA viruses by nonisotopic dot\u2010blot hybridization","volume":"81","author":"Pallas V.","year":"1998","journal-title":"Methods Mol. 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