{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:19:09Z","timestamp":1762251549427,"version":"build-2065373602"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,7,15]],"date-time":"2016-07-15T00:00:00Z","timestamp":1468540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Spanish Ministry of Economy and Competitivity","award":["AGL2014-52871-R,"],"award-info":[{"award-number":["AGL2014-52871-R,"]}]},{"name":"European Regional Development Funds"}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    In this work, we investigated the involvement of the long\u2010term dynamics of cytoskeletal reorganization on the induced inaccessibility phenomenon by which cells that successfully defend against a previous fungal attack become highly resistant to subsequent attacks. This was performed on pea through double inoculation experiments using inappropriate (\n                    <jats:italic>Blumeria graminis<\/jats:italic>\n                    f. sp\n                    <jats:italic>. avenae<\/jats:italic>\n                    ,\n                    <jats:italic>Bga<\/jats:italic>\n                    ) and appropriate (\n                    <jats:italic>Erysiphe pisi<\/jats:italic>\n                    ,\n                    <jats:italic>Ep<\/jats:italic>\n                    ) powdery mildew fungi. Pea leaves previously inoculated with\n                    <jats:italic>Bga<\/jats:italic>\n                    showed a significant reduction of later\n                    <jats:italic>Ep<\/jats:italic>\n                    infection relative to leaves inoculated only with\n                    <jats:italic>Ep<\/jats:italic>\n                    , indicating that cells had developed induced inaccessibility. This reduction in\n                    <jats:italic>Ep<\/jats:italic>\n                    infection was higher when the time interval between\n                    <jats:italic>Bga<\/jats:italic>\n                    and\n                    <jats:italic>Ep<\/jats:italic>\n                    inoculation ranged between 18 and 24 h, although increased penetration resistance in co\u2010infected cells was observed even with time intervals of 24 days between inoculations. Interestingly, this increase in resistance to\n                    <jats:italic>Ep<\/jats:italic>\n                    following successful defence to the inappropriate\n                    <jats:italic>Bga<\/jats:italic>\n                    was associated with an increase in actin microfilament density that reached a maximum at 18\u201324 h after\n                    <jats:italic>Bga<\/jats:italic>\n                    inoculation and very slowly decreased afterwards. The putative role of cytoskeleton reorganization\/disorganization leading to inaccessibility is supported by the suppression of the induced resistance mediated by specific actin (cytochalasin D, latrunculin B) or general protein (cycloheximide) inhibitors.\n                  <\/jats:p>","DOI":"10.1111\/mpp.12424","type":"journal-article","created":{"date-parts":[[2016,5,5]],"date-time":"2016-05-05T04:49:29Z","timestamp":1462423769000},"page":"662-671","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Cytoskeleton reorganization\/disorganization is a key feature of induced inaccessibility for defence to successive pathogen attacks"],"prefix":"10.1111","volume":"18","author":[{"given":"Juan","family":"Moral","sequence":"first","affiliation":[{"name":"CSIC, Institute for Sustainable Agriculture C\u00f3rdoba E\u201014004 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