{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T19:34:44Z","timestamp":1790451284249,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,10,12]],"date-time":"2016-10-12T00:00:00Z","timestamp":1476230400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Spanish Ministry of Science and Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    RNA silencing is an ancient regulatory mechanism operating in all eukaryotic cells. In fungi, it was first discovered in\n                    <jats:italic>Neurospora crassa<\/jats:italic>\n                    , although its potential as a defence mechanism against mycoviruses was first reported in\n                    <jats:italic>Cryphonectria parasitica<\/jats:italic>\n                    and, later, in several fungal species. There is little evidence of the antiviral potential of RNA silencing in the phytopathogenic species of the fungal genus\n                    <jats:italic>Botrytis<\/jats:italic>\n                    . Moreover, little is known about the RNA silencing components in these fungi, although the analysis of public genome databases identified two Dicer\u2010like genes in\n                    <jats:italic>B. cinerea<\/jats:italic>\n                    , as in most of the ascomycetes sequenced to date. In this work, we used deep sequencing to study the virus\u2010derived small RNA (vsiRNA) populations from different mycoviruses infecting field isolates of\n                    <jats:italic>Botrytis<\/jats:italic>\n                    spp. The mycoviruses under study belong to different genera and species, and have different types of genome [double\u2010stranded RNA (dsRNA), (+)single\u2010stranded RNA (ssRNA) and (\u2013)ssRNA]. In general, vsiRNAs derived from mycoviruses are mostly of 21, 20 and 22 nucleotides in length, possess sense or antisense orientation, either in a similar ratio or with a predominance of sense polarity depending on the virus species, have predominantly U at their 5\u2032 end, and are unevenly distributed along the viral genome, showing conspicuous hotspots of vsiRNA accumulation. These characteristics reveal striking similarities with vsiRNAs produced by plant viruses, suggesting similar pathways of viral targeting in plants and fungi. We have shown that the fungal RNA silencing machinery acts against the mycoviruses used in this work in a similar manner independent of their viral or fungal origin.\n                  <\/jats:p>","DOI":"10.1111\/mpp.12466","type":"journal-article","created":{"date-parts":[[2016,8,31]],"date-time":"2016-08-31T02:52:58Z","timestamp":1472611978000},"page":"1127-1137","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Deep sequencing of mycovirus\u2010derived small RNAs from\n                    <i>Botrytis<\/i>\n                    species"],"prefix":"10.1111","volume":"18","author":[{"given":"Livia","family":"Donaire","sequence":"first","affiliation":[{"name":"Centro de Investigaciones Biol\u00f3gicas Consejo Superior de Investigaciones Cient\u00edficas (CIB\u2010CSIC) Madrid 28040 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda A.","family":"Ayll\u00f3n","sequence":"additional","affiliation":[{"name":"Centro de Biotecnolog\u00eda y Gen\u00f3mica de Plantas Universidad Polit\u00e9cnica de Madrid (UPM)\u2010Instituto Nacional de Investigaci\u00f3n Agraria y Alimentaria (INIA), Campus de Montegancedo, Pozuelo de Alarc\u00f3n  Madrid 28223 Spain"},{"name":"Departamento Biotecnolog\u00eda\u2010Biolog\u00eda Vegetal E.T.S.I. Agron\u00f3mica, Alimentaria y de Biosistemas, UPM Madrid 28040 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,10,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-010-0869-8"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020746"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1138030"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0042758"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069283"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2179"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1509151112"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00557-13"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.02.028"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2011.10.028"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.07.039"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.07.005"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.11.027"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/35888"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.02.034"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00356-07"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.10.008"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-82-1-67"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.05.027"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2013.03.002"},{"key":"e_1_2_6_22_1","volume-title":"Virus Taxonomy. 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