{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T07:52:13Z","timestamp":1792223533530,"version":"4.4.0"},"reference-count":65,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,11,21]],"date-time":"2017-11-21T00:00:00Z","timestamp":1511222400000},"content-version":"am","delay-in-days":303,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2017,1,22]],"date-time":"2017-01-22T00:00:00Z","timestamp":1485043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IOS\u20100842716"],"award-info":[{"award-number":["IOS\u20100842716"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    DEFORMED ROOT AND LEAVES1 (DRL1) is an Arabidopsis homologue of the yeast TOXIN TARGET4 (TOT4)\/KILLER TOXIN\u2010INSENSITIVE12 (KTI12) protein that is physically associated with the RNA polymerase II\u2010interacting protein complex named Elongator. Mutations in DRL1 and Elongator lead to similar morphological and molecular phenotypes, suggesting that DRL1 and Elongator may functionally overlap in Arabidopsis. We have shown previously that Elongator plays an important role in both salicylic acid (SA)\u2010 and jasmonic acid (JA)\/ethylene (ET)\u2010mediated defence responses. Here, we tested whether DRL1 also plays a similar role as Elongator in plant immune responses. Our results show that, although DRL1 partially contributes to SA\u2010induced cytotoxicity, it does not play a significant role in SA\u2010mediated expression of\n                    <jats:italic>PATHOGENESIS\u2010RELATED<\/jats:italic>\n                    genes and resistance to the virulent bacterial pathogen\n                    <jats:italic>Pseudomonas syringae<\/jats:italic>\n                    pv.\n                    <jats:italic>maculicola<\/jats:italic>\n                    ES4326. In contrast, DRL1 is required for JA\/ET\u2010 and necrotrophic fungal pathogen\n                    <jats:italic>Botrytis cinerea<\/jats:italic>\n                    \u2010induced defence gene expression and for resistance to\n                    <jats:italic>B.\u00a0cinerea<\/jats:italic>\n                    and\n                    <jats:italic>Alternaria brassicicola<\/jats:italic>\n                    . Furthermore, unlike the\n                    <jats:italic>TOT4<\/jats:italic>\n                    \/\n                    <jats:italic>KTI12<\/jats:italic>\n                    gene which, when overexpressed in yeast, confers zymocin resistance, a phenotype of the\n                    <jats:italic>tot4<\/jats:italic>\n                    \/\n                    <jats:italic>kti12<\/jats:italic>\n                    mutant, overexpression of\n                    <jats:italic>DRL1<\/jats:italic>\n                    does not change\n                    <jats:italic>B.\u00a0cinerea<\/jats:italic>\n                    \u2010induced defence gene expression and resistance to this pathogen. Finally, DRL1 contains an N\u2010terminal P\u2010loop and a C\u2010terminal calmodulin (CaM)\u2010binding domain and is a CaM\u2010binding protein. We demonstrate that both the P\u2010loop and the CaM\u2010binding domain are essential for the function of DRL1 in\n                    <jats:italic>B.\u00a0cinerea<\/jats:italic>\n                    \u2010induced expression of\n                    <jats:italic>PDF1.2<\/jats:italic>\n                    and\n                    <jats:italic>ORA59<\/jats:italic>\n                    , and in resistance to\n                    <jats:italic>B.\u00a0cinerea<\/jats:italic>\n                    , suggesting that the function of DRL1 in plant immunity may be regulated by ATP\/GTP and CaM binding.\n                  <\/jats:p>","DOI":"10.1111\/mpp.12516","type":"journal-article","created":{"date-parts":[[2016,11,21]],"date-time":"2016-11-21T02:46:48Z","timestamp":1479696408000},"page":"286-299","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The Elongator complex\u2010associated protein DRL1 plays a positive role in immune responses against necrotrophic fungal pathogens in Arabidopsis"],"prefix":"10.1111","volume":"19","author":[{"given":"Chenggang","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Microbiology and Cell Science University of Florida, PO Box 110700 Gainesville FL 32611 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xudong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science University of Florida, PO Box 110700 Gainesville FL 32611 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Liang","family":"Li","sequence":"additional","affiliation":[{"name":"Sanford Burnham Prebys Medical Discovery Institute at Lake Nona Orlando FL 32827 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Interdisciplinary Center for Biotechnology Research, University of Florida, PO Box 103622 Gainesville FL 32610 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhonglin","family":"Mou","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science University of Florida, PO Box 110700 Gainesville FL 32611 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2006.02849.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1086391"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.44.070505.143440"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.9.6306"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2307\/3869945"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81858-9"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00433-06"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1998.00343.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.11.043"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2010.04345.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-13-102"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.14.6602"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2004.07.005"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07612"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2006.07.031"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2006.02999.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.095596"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02794.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.8.1993"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.43.040204.135923"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2008.00346.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110445200"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.7247705"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erv230"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.14348\/molcells.2015.2297"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.115717"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.60.070191.002025"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.12.12.2339"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2005.02508.x"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.111.090571"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2007.08.008"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2006.02.005"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.007468"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90282-8"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1962.tb08052.x"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.007062"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0502600102"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0913559107"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.192310"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.105.034819"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08732"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80179-3"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413373200"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.164"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.113.220780"},{"key":"e_1_2_7_48_1","first-page":"211","article-title":"Jasmonate\u2010signals in plant\u2013microbe interactions","volume":"23","author":"Pozo M.J.","year":"2005","journal-title":"J. Plant Growth Regul."},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.108.117523"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2005.02.018"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm412"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000861"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.1997.10.1.69"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.2202\/1544-6115.1027"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(00)00183-7"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.050908.135202"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.12946"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000301"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.113.109116"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.102046"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.022042899"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80194-X"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2011.04831.x"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.103317"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2006.02901.x"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2009.03931.x"}],"container-title":["Molecular Plant Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmpp.12516","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mpp.12516","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mpp.12516","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/am-pdf\/10.1111\/mpp.12516","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mpp.12516","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mpp.12516","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:58:37Z","timestamp":1762275517000},"score":1,"resource":{"primary":{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/10.1111\/mpp.12516"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,22]]},"references-count":65,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1111\/mpp.12516"],"URL":"https:\/\/doi.org\/10.1111\/mpp.12516","archive":["Portico"],"relation":{},"ISSN":["1464-6722","1364-3703"],"issn-type":[{"value":"1464-6722","type":"print"},{"value":"1364-3703","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,22]]},"assertion":[{"value":"2016-08-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-11-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-01-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}