{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T18:40:23Z","timestamp":1790361623839,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T00:00:00Z","timestamp":1486512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006580","name":"Office of the Chief Scientist, Ministry of Health","doi-asserted-by":"publisher","award":["132165015"],"award-info":[{"award-number":["132165015"]}],"id":[{"id":"10.13039\/501100006580","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006031","name":"United States - Israel Binational Agricultural Research and Development Fund","doi-asserted-by":"publisher","award":["Grant\u2010IS\u20104513\u201012"],"award-info":[{"award-number":["Grant\u2010IS\u20104513\u201012"]}],"id":[{"id":"10.13039\/100006031","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    RNA\u2010dependent RNA polymerase 1 (RDR1) plays a crucial role in plant defence against viruses. In this study, it was observed that cucumber,\n                    <jats:italic>Cucumis sativus<\/jats:italic>\n                    , uniquely encodes a small gene family of four\n                    <jats:italic>RDR1<\/jats:italic>\n                    genes. The cucumber\n                    <jats:italic>RDR1<\/jats:italic>\n                    genes (\n                    <jats:italic>CsRDR1a<\/jats:italic>\n                    ,\n                    <jats:italic>CsRDR1b<\/jats:italic>\n                    and duplicated\n                    <jats:italic>CsRDR1c1\/c2<\/jats:italic>\n                    ) shared 55%\u201360% homology in their encoded amino acid sequences. In healthy cucumber plants,\n                    <jats:italic>RDR1a<\/jats:italic>\n                    and\n                    <jats:italic>RDR1b<\/jats:italic>\n                    transcripts were expressed at higher levels than transcripts of\n                    <jats:italic>RDR1c1\/c2<\/jats:italic>\n                    , which were barely detectable. The expression of all four\n                    <jats:italic>CsRDR1<\/jats:italic>\n                    genes was induced by virus infection, after which the expression level of\n                    <jats:italic>CsRDR1b<\/jats:italic>\n                    increased 10\u201320\u2010fold in several virus\u2010resistant cucumber cultivars and in a broad virus\u2010resistant transgenic cucumber line expressing a high level of transgene small RNAs, all without alteration in salicylic acid (SA) levels. By comparison,\n                    <jats:italic>CsRDR1c1\/c2<\/jats:italic>\n                    genes were highly induced (25\u20131300\u2010fold) in susceptible cucumber cultivars infected with RNA or DNA viruses. Inhibition of\n                    <jats:italic>RDR1c1<\/jats:italic>\n                    \/c2 expression led to increased virus accumulation. Ectopic application of SA induced the expression of cucumber\n                    <jats:italic>RDR1a<\/jats:italic>\n                    ,\n                    <jats:italic>RDR1b<\/jats:italic>\n                    and\n                    <jats:italic>RDRc1\/c2<\/jats:italic>\n                    genes. A constitutive high level of\n                    <jats:italic>RDR1b<\/jats:italic>\n                    gene expression independent of SA was found to be associated with broad virus resistance. These findings show that multiple\n                    <jats:italic>RDR1<\/jats:italic>\n                    genes are involved in virus resistance in cucumber and are regulated in a coordinated fashion with different expression profiles.\n                  <\/jats:p>","DOI":"10.1111\/mpp.12518","type":"journal-article","created":{"date-parts":[[2016,11,23]],"date-time":"2016-11-23T10:06:42Z","timestamp":1479895602000},"page":"300-312","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Differential expression of cucumber RNA\u2010dependent RNA polymerase 1 genes during antiviral defence and resistance"],"prefix":"10.1111","volume":"19","author":[{"given":"Diana","family":"Leibman","sequence":"first","affiliation":[{"name":"Department of Plant Pathology and Weed Research Agricultural Research Organization, Volcani Center Bet Dagan 50250 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Kravchik","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Weed Research Agricultural Research Organization, Volcani Center Bet Dagan 50250 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalia","family":"Wolf","sequence":"additional","affiliation":[{"name":"Department of Vegetable and Field Crops Agricultural Research Organization, Volcani Center Bet Dagan 50250 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Haviv","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Weed Research Agricultural Research Organization, Volcani Center Bet Dagan 50250 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mira","family":"Weissberg","sequence":"additional","affiliation":[{"name":"Department of Fruit Tree Sciences Agricultural Research Organization, Volcani Center Bet Dagan 50250 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ron","family":"Ophir","sequence":"additional","affiliation":[{"name":"Department of Fruit Tree Sciences Agricultural Research Organization, Volcani Center Bet Dagan 50250 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry S.","family":"Paris","sequence":"additional","affiliation":[{"name":"Department of Vegetable Crops and Plant Genetics Agricultural Research Organization, Newe Ya'ar Research Center, PO Box 1021 Ramat Yishay 30\u2010095 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Palukaitis","sequence":"additional","affiliation":[{"name":"Department of Horticultural Sciences Seoul Women's University Nowon\u2010gu Seoul 01797 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shou\u2010Wei","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Microbiology &amp; Institute for Integrative Genome Biology University of California Riverside, CA 92521 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Gaba","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Weed Research Agricultural Research Organization, Volcani Center Bet Dagan 50250 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Gal\u2010On","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Weed Research Agricultural Research Organization, Volcani Center Bet Dagan 50250 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.67.4.657-685.2003"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2006.02861.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1656(01)00229-2"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02874"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1407131111"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3527(10)76003-6"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80864-8"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02712670"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.106.047449"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.07.039"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00272-08"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2007.00381.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00344-015-9514-9"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.109.073056"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.102.017640"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/10635150390235520"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5441.950"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066530"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep23082"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.2000.13.3.347"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.199646"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-05-11-0128"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2008.12.017"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2009.03.003"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-18-0428"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02873"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2007.03030.x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0711363105"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcr281"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004971"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-06-10-0124"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0805760105"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-22-10-1312"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)50278-2"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2005.11.017"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv318"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.12.1246"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.106.082107"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.10.054"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-06-10-0159"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1733874100"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003399"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.15.01283"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-14-177"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0904086107"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-003-0240-4"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/35081168"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1199\/tab.0146"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.111440998"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-013-1863-7"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0304346101"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.109.072058"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.2003.16.3.206"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-11-13-0349-R"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2009.07.004"}],"container-title":["Molecular Plant Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmpp.12518","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mpp.12518","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mpp.12518","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mpp.12518","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:58:05Z","timestamp":1762275485000},"score":1,"resource":{"primary":{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/10.1111\/mpp.12518"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,8]]},"references-count":55,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1111\/mpp.12518"],"URL":"https:\/\/doi.org\/10.1111\/mpp.12518","archive":["Portico"],"relation":{},"ISSN":["1464-6722","1364-3703"],"issn-type":[{"value":"1464-6722","type":"print"},{"value":"1364-3703","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,8]]},"assertion":[{"value":"2016-07-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-11-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}