{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T11:55:45Z","timestamp":1792324545712,"version":"4.4.0"},"reference-count":74,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,8,10]],"date-time":"2017-08-10T00:00:00Z","timestamp":1502323200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union's Seventh Framework Programme for research, technological development, and demonstration","award":["EU GA-2013-608422-IDP BRIDGES"],"award-info":[{"award-number":["EU GA-2013-608422-IDP BRIDGES"]}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Cassava brown streak virus<\/jats:italic>\n                    (CBSV) and\n                    <jats:italic>Ugandan cassava brown streak virus<\/jats:italic>\n                    (UCBSV) are responsible for significant cassava yield losses in eastern sub\u2010Saharan Africa. To study the possible mechanisms of plant resistance to CBSVs, we inoculated CBSV\u2010susceptible and CBSV\u2010resistant cassava varieties with a mixed infection of CBSVs using top\u2010cleft grafting. Transcriptome profiling of the two cassava varieties was performed at the earliest time point of full infection (28\u00a0days after grafting) in the susceptible scions. The expression of genes encoding proteins in RNA silencing, salicylic acid pathways and callose deposition was altered in the susceptible cassava variety, but transcriptional changes were limited in the resistant variety. In total, the expression of 585 genes was altered in the resistant variety and 1292 in the susceptible variety. Transcriptional changes led to the activation of \u03b2\u20101,3\u2010glucanase enzymatic activity and a reduction in callose deposition in the susceptible cassava variety. Time course analysis also showed that CBSV replication in susceptible cassava induced a strong up\u2010regulation of\n                    <jats:italic>RDR1<\/jats:italic>\n                    , a gene previously reported to be a susceptibility factor in other potyvirus\u2013host pathosystems. The differences in the transcriptional responses to CBSV infection indicated that susceptibility involves the restriction of callose deposition at plasmodesmata. Aniline blue staining of callose deposits also indicated that the resistant variety displays a moderate, but significant, increase in callose deposition at the plasmodesmata. Transcriptome data suggested that resistance does not involve typical antiviral defence responses (i.e. RNA silencing and salicylic acid). A meta\u2010analysis of the current RNA\u2010sequencing (RNA\u2010seq) dataset and selected potyvirus\u2013host and virus\u2013cassava RNA\u2010seq datasets revealed that the conservation of the host response across pathosystems is restricted to genes involved in developmental processes.\n                  <\/jats:p>","DOI":"10.1111\/mpp.12565","type":"journal-article","created":{"date-parts":[[2017,5,11]],"date-time":"2017-05-11T19:36:44Z","timestamp":1494531404000},"page":"476-489","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Molecular insights into\n                    <i>Cassava brown streak virus<\/i>\n                    susceptibility and resistance by profiling of the early host response"],"prefix":"10.1111","volume":"19","author":[{"given":"Ravi B.","family":"Anjanappa","sequence":"first","affiliation":[{"name":"Department of Biology ETH Zurich 8092 Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devang","family":"Mehta","sequence":"additional","affiliation":[{"name":"Department of Biology ETH Zurich 8092 Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal J.","family":"Okoniewski","sequence":"additional","affiliation":[{"name":"ID Scientific IT Services ETH Zurich 8092 Zurich Switzerland"},{"name":"Functional Genomics Center Zurich 8057 Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicja","family":"Szabelska\u2010Ber\u0229sewicz","sequence":"additional","affiliation":[{"name":"Functional Genomics Center Zurich 8057 Zurich Switzerland"},{"name":"Department of Mathematical and Statistical Methods Poznan University of Life Sciences 60\u2010637 Poznan Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilhelm","family":"Gruissem","sequence":"additional","affiliation":[{"name":"Department of Biology ETH Zurich 8092 Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Vanderschuren","sequence":"additional","affiliation":[{"name":"Department of Biology ETH Zurich 8092 Zurich Switzerland"},{"name":"AgroBioChem Department, Gembloux Agro\u2010Bio Tech University of Li\u00e8ge 5030 Gembloux 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