{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T12:06:19Z","timestamp":1788437179493,"version":"build-2803163510"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,11,29]],"date-time":"2017-11-29T00:00:00Z","timestamp":1511913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Cooperative Research Program for Agriculture Science & Technology Development","award":["PJ01250602"],"award-info":[{"award-number":["PJ01250602"]}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The Gac\/Rsm network regulates, at the transcriptional level, many beneficial traits in biocontrol\u2010active pseudomonads. In this study, we used Phenotype MicroArrays, followed by specific growth studies and mutational analysis, to understand how catabolism is regulated by this sensor kinase system in the biocontrol isolate\n                    <jats:italic>Pseudomonas chlororaphis<\/jats:italic>\n                    O6. The growth of a\n                    <jats:italic>gacS<\/jats:italic>\n                    mutant was decreased significantly relative to that of the wild\u2010type on ornithine and arginine, and on the precursor of these amino acids,\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetyl\u2010\n                    <jats:sc>l<\/jats:sc>\n                    \u2010glutamic acid. The\n                    <jats:italic>gacS<\/jats:italic>\n                    mutant also showed reduced production of polyamines. Expression of the genes encoding arginine decarboxylase (\n                    <jats:italic>speA<\/jats:italic>\n                    ) and ornithine decarboxylases (\n                    <jats:italic>speC<\/jats:italic>\n                    ) was controlled at the transcriptional level by the GacS sensor of\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>chlororaphis<\/jats:italic>\n                    O6. Polyamine production was reduced in the\n                    <jats:italic>speC<\/jats:italic>\n                    mutant, and was eliminated in the speAspeC mutant. The addition of exogenous polyamines to the s\n                    <jats:italic>peAspeC<\/jats:italic>\n                    mutant restored the\n                    <jats:italic>in vitro<\/jats:italic>\n                    growth inhibition of two fungal pathogens, as well as the secretion of three biological control\u2010related factors: pyrrolnitrin, protease and siderophore. These results extend our knowledge of the regulation by the Gac\/Rsm network in a biocontrol pseudomonad to include polyamine synthesis. Collectively, our studies demonstrate that bacterial polyamines act as important regulators of bacterial cell growth and biocontrol potential.\n                  <\/jats:p>","DOI":"10.1111\/mpp.12610","type":"journal-article","created":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T10:22:41Z","timestamp":1504261361000},"page":"1257-1266","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Polyamine is a critical determinant of\n                    <i>Pseudomonas chlororaphis<\/i>\n                    O6 for GacS\u2010dependent bacterial cell growth and biocontrol capacity"],"prefix":"10.1111","volume":"19","author":[{"given":"Ju Yeon","family":"Park","sequence":"first","affiliation":[{"name":"Department of Applied Biology College of Agriculture and Life Sciences, Chonnam National University Gwangju 61186 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beom Ryong","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Applied Biology College of Agriculture and Life Sciences, Chonnam National University Gwangju 61186 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Choong\u2010Min","family":"Ryu","sequence":"additional","affiliation":[{"name":"Molecular Phytobacteriology Laboratory Infectious Disease Research Center, KRIBB Daejeon 34141 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne J.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Utah State University Logan UT 84322\u20105305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7661-7600","authenticated-orcid":false,"given":"Young Cheol","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Applied Biology College of Agriculture and Life Sciences, Chonnam National University Gwangju 61186 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06383.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2005.00912.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1014"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.7.3308-3314.2002"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-02-17-0027-R"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1758-2229.12061"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:MCBI.0000038227.91813.79"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02557-14"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00679-11"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.8.3142-3150.2000"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2016.03.008"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-015-9906-0"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-19-0924"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.02134.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01528.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.9.3464"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2009.42"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036149"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2003.10.004"},{"key":"e_1_2_8_21_1","first-page":"586","article-title":"Inhibition of seed germination and induction of systemic disease resistance by Pseudomonas chlororaphis O6 requires phenazine production regulated by the global regulator, GacS","volume":"17","author":"Kang B.R.","year":"2007","journal-title":"J. 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