{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:41:28Z","timestamp":1790700088259,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,1,30]],"date-time":"2018-01-30T00:00:00Z","timestamp":1517270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Scientific Research Foundation for the Introduced Talents of Shenyang Agricultural University","award":["20153040"],"award-info":[{"award-number":["20153040"]}]},{"DOI":"10.13039\/501100012669","name":"Natural Science Foundation Project of CQ CSTC","doi-asserted-by":"crossref","award":["2011BB1101"],"award-info":[{"award-number":["2011BB1101"]}],"id":[{"id":"10.13039\/501100012669","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The fungus\n                    <jats:italic>Sclerotinia sclerotiorum<\/jats:italic>\n                    is a necrotrophic plant pathogen causing significant damage on a broad range of crops. This fungus produces sclerotia that serve as the long\u2010term survival structures in the life cycle and the primary inoculum in the disease cycle. Melanin plays an important role in protecting mycelia and sclerotia from ultraviolet radiation and other adverse environmental conditions. In this study, two genes,\n                    <jats:italic>SCD1<\/jats:italic>\n                    encoding a scytalone dehydratase and\n                    <jats:italic>THR1<\/jats:italic>\n                    encoding a trihydroxynaphthalene reductase, were disrupted by target gene replacement, and their roles in mycelial growth, sclerotial development and fungal pathogenicity were investigated. Phylogenetic analyses indicated that the deduced amino acid sequences of\n                    <jats:italic>SCD1<\/jats:italic>\n                    and\n                    <jats:italic>THR1<\/jats:italic>\n                    were similar to the orthologues of\n                    <jats:italic>Botrytis cinerea<\/jats:italic>\n                    . Expression of\n                    <jats:italic>SCD1<\/jats:italic>\n                    was at higher levels in sclerotia relative to mycelia.\n                    <jats:italic>THR1<\/jats:italic>\n                    was expressed at similar levels in mycelia and sclerotia at early stages, but was up\u2010regulated in sclerotia at the maturation stage. Disruption of\n                    <jats:italic>SCD1<\/jats:italic>\n                    or\n                    <jats:italic>THR1<\/jats:italic>\n                    did not change the pathogenicity of the fungus, but resulted in slower radial growth, less biomass, wider angled hyphal branches, impaired sclerotial development and decreased resistance to ultraviolet light.\n                  <\/jats:p>","DOI":"10.1111\/mpp.12627","type":"journal-article","created":{"date-parts":[[2017,10,11]],"date-time":"2017-10-11T08:52:35Z","timestamp":1507711955000},"page":"1444-1453","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":71,"title":["Deficiency of the melanin biosynthesis genes\n                    <i>SCD1<\/i>\n                    and\n                    <i>THR1<\/i>\n                    affects sclerotial development and vegetative growth, but not pathogenicity, in\n                    <i>Sclerotinia sclerotiorum<\/i>"],"prefix":"10.1111","volume":"19","author":[{"given":"Yue","family":"Liang","sequence":"first","affiliation":[{"name":"College of Plant Protection Shenyang Agricultural University Shenyang Liaoning 110866 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Xiong","sequence":"additional","affiliation":[{"name":"School of Life Sciences Chongqing University Chongqing 400045 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siegrid","family":"Steinkellner","sequence":"additional","affiliation":[{"name":"Division of Plant Protection, Department of Crop Sciences University of Natural Resources and Life Sciences Vienna Vienna 1190 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Feng","sequence":"additional","affiliation":[{"name":"Alberta Plant Health Laboratory, Alberta Agriculture and Forestry Edmonton Alberta T5Y 6H3 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