{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:53:04Z","timestamp":1772160784615,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,12,26]],"date-time":"2017-12-26T00:00:00Z","timestamp":1514246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002661","name":"Fonds De La Recherche Scientifique - FNRS","doi-asserted-by":"publisher","award":["T.0006.14"],"award-info":[{"award-number":["T.0006.14"]}],"id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004385","name":"Universiteit Gent","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004385","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Common scab disease on root and tuber plants is caused by\n                    <jats:italic>Streptomyces scabies<\/jats:italic>\n                    and related species which use the cellulose synthase inhibitor thaxtomin A as the main phytotoxin. Thaxtomin production is primarily triggered by the import of cello\u2010oligosaccharides. Once inside the cell, the fate of the cello\u2010oligosaccharides is dichotomized: (i) the fuelling of glycolysis with glucose for the saprophytic lifestyle through the action of \u03b2\u2010glucosidase(s) (BGs); and (ii) elicitation of the pathogenic lifestyle by the inhibition of CebR\u2010mediated transcriptional repression of thaxtomin biosynthetic genes. Here, we investigated the role of\n                    <jats:italic>scab57721<\/jats:italic>\n                    , encoding a putative BG (BglC), in the onset of the pathogenicity of\n                    <jats:italic>S.\u00a0scabies<\/jats:italic>\n                    . Enzymatic assays showed that BglC was able to release glucose from cellobiose, cellotriose and all other cello\u2010oligosaccharides tested. Its inactivation resulted in a phenotype opposite to that expected, as reduced production of thaxtomin was monitored when the mutant was cultivated on medium containing cello\u2010oligosaccharides as unique carbon source. This unexpected phenotype could be attributed to the highly increased activity of alternative intracellular BGs, probably as a compensation for\n                    <jats:italic>bglC<\/jats:italic>\n                    inactivation, which then prevented cellobiose and cellotriose accumulation to reduce the activity of CebR. In contrast, when the\n                    <jats:italic>bglC<\/jats:italic>\n                    null mutant was cultivated on medium devoid of cello\u2010oligosaccharides, it instead constitutively produced thaxtomin. This observed hypervirulent phenotype does not fit with the proposed model of the cello\u2010oligosaccharide\u2010mediated induction of thaxtomin production, and suggests that the role of BglC in the route to the pathogenic lifestyle of\n                    <jats:italic>S.\u00a0scabies<\/jats:italic>\n                    is more complex than currently presented.\n                  <\/jats:p>","DOI":"10.1111\/mpp.12631","type":"journal-article","created":{"date-parts":[[2017,10,27]],"date-time":"2017-10-27T08:52:42Z","timestamp":1509094362000},"page":"1480-1490","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Contribution of the \u03b2\u2010glucosidase BglC to the onset of the pathogenic lifestyle of\n                    <i>Streptomyces scabies<\/i>"],"prefix":"10.1111","volume":"19","author":[{"given":"Samuel","family":"Jourdan","sequence":"first","affiliation":[{"name":"InBioS \u2013 Center for Protein Engineering University of Li\u00e8ge, Institut de Chimie Li\u00e8ge B\u20104000 Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isolde M.","family":"Francis","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology University of Florida Gainesville 32611 FL USA"},{"name":"Department of Biology California State University Bakersfield 93311 CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benoit","family":"Deflandre","sequence":"additional","affiliation":[{"name":"InBioS \u2013 Center for Protein Engineering University of Li\u00e8ge, Institut de Chimie Li\u00e8ge B\u20104000 Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elodie","family":"Tenconi","sequence":"additional","affiliation":[{"name":"InBioS \u2013 Center for Protein Engineering University of Li\u00e8ge, Institut de Chimie Li\u00e8ge B\u20104000 Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Riley","sequence":"additional","affiliation":[{"name":"Department of Biology California State University Bakersfield 93311 CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00f6ren","family":"Planckaert","sequence":"additional","affiliation":[{"name":"Laboratory for Protein Biochemistry and Biomolecular Engineering, Department of Biochemistry and Microbiology Ghent University Ghent B\u20109000 Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Tocquin","sequence":"additional","affiliation":[{"name":"InBioS \u2013 CARE PhytoSYSTEMS, University of Li\u00e8ge, Institut de Botanique Li\u00e8ge B\u20104000 Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lo\u00efc","family":"Martinet","sequence":"additional","affiliation":[{"name":"InBioS \u2013 Center for Protein Engineering University of Li\u00e8ge, Institut de Chimie Li\u00e8ge B\u20104000 Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bart","family":"Devreese","sequence":"additional","affiliation":[{"name":"Laboratory for Protein Biochemistry and Biomolecular Engineering, Department of Biochemistry and Microbiology Ghent University Ghent B\u20109000 Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosemary","family":"Loria","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology University of Florida Gainesville 32611 FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e9bastien","family":"Rigali","sequence":"additional","affiliation":[{"name":"InBioS \u2013 Center for Protein Engineering University of Li\u00e8ge, Institut de Chimie Li\u00e8ge B\u20104000 Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.4.1529-1541.2005"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-010-9429-1"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02018-14"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02194-14"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0337542100"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2800309"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmpp.2007.09.003"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05942.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep27144"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00367-16"},{"key":"e_1_2_6_12_1","volume-title":"Practical 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