{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T01:48:45Z","timestamp":1785289725805,"version":"3.55.0"},"reference-count":54,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,5,2]],"date-time":"2018-05-02T00:00:00Z","timestamp":1525219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["BEX 6948\/10\u20103"],"award-info":[{"award-number":["BEX 6948\/10\u20103"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["486206\/2012\u20102"],"award-info":[{"award-number":["486206\/2012\u20102"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["312991\/2013\u20104"],"award-info":[{"award-number":["312991\/2013\u20104"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Terpene volatiles play an important role in the interactions between specialized pathogens and fruits. Citrus black spot (CBS), caused by the fungus\n                    <jats:italic>Phyllosticta citricarpa<\/jats:italic>\n                    , is associated with crop losses in different citrus\u2010growing areas worldwide. The pathogen may infect the fruit for 20\u201324\u00a0weeks after petal fall, but the typical hard spot symptoms appear when the fruit have almost reached maturity, caused by fungal colonization and the induction of cell lysis around essential oil cavities.\n                    <jats:sc>d<\/jats:sc>\n                    \u2010Limonene represents approximately 95% of the total oil gland content in mature orange fruit. Herein, we investigated whether orange fruit with reduced\n                    <jats:sc>d<\/jats:sc>\n                    \u2010limonene content in peel oil glands via an antisense (AS) approach may affect fruit interaction with\n                    <jats:italic>P.\u00a0citricarpa<\/jats:italic>\n                    relative to empty vector (EV) controls. AS fruit showed enhanced resistance to the fungus relative to EV fruit. Because of the reduced\n                    <jats:sc>d<\/jats:sc>\n                    \u2010limonene content, an over\u2010accumulation of linalool and other monoterpene alcohols was found in AS relative to EV fruit. A global gene expression analysis at 2\u00a0h and 8\u00a0days after inoculation with\n                    <jats:italic>P.\u00a0citricarpa<\/jats:italic>\n                    revealed the activation of defence responses in AS fruit via the up\u2010regulation of different pathogenesis\u2010related (PR) protein genes, probably as a result of enhanced constitutive accumulation of linalool and other alcohols. When assayed\n                    <jats:italic>in\u00a0vitro<\/jats:italic>\n                    and\n                    <jats:italic>in\u00a0vivo<\/jats:italic>\n                    , monoterpene alcohols at the concentrations present in AS fruit showed strong antifungal activity. We show here that terpene engineering in fruit peels could be a promising method for the development of new strategies to obtain resistance to fruit diseases.\n                  <\/jats:p>","DOI":"10.1111\/mpp.12681","type":"journal-article","created":{"date-parts":[[2018,3,24]],"date-time":"2018-03-24T19:38:28Z","timestamp":1521920308000},"page":"2077-2093","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Engineering\n                    <scp>d<\/scp>\n                    \u2010limonene synthase down\u2010regulation in orange fruit induces resistance against the fungus\n                    <i>Phyllosticta citricarpa<\/i>\n                    through enhanced accumulation of monoterpene alcohols and activation of defence"],"prefix":"10.1111","volume":"19","author":[{"given":"Ana","family":"Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia Vegetal, Fundo de Defesa da Citricultura (Fundecitrus) Araraquara S\u00e3o Paulo 14807\u2013040 Brazil"},{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cient\u00edficas (IBMCP\u2010CSIC) Valencia 46022 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Kava","sequence":"additional","affiliation":[{"name":"Depto. de Gen\u00e9tica, Universidade Federal do Paran\u00e1 Curitiba Paran\u00e1 81.531\u2010980 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena","family":"Latorre\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cient\u00edficas (IBMCP\u2010CSIC) Valencia 46022 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Geraldo J.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia Vegetal, Fundo de Defesa da Citricultura (Fundecitrus) Araraquara S\u00e3o Paulo 14807\u2013040 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosana G.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia Vegetal, Fundo de Defesa da Citricultura (Fundecitrus) Araraquara S\u00e3o Paulo 14807\u2013040 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chirlei","family":"Glienke","sequence":"additional","affiliation":[{"name":"Depto. de Gen\u00e9tica, Universidade Federal do Paran\u00e1 Curitiba Paran\u00e1 81.531\u2010980 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisandra S.","family":"Ferreira\u2010Maba","sequence":"additional","affiliation":[{"name":"Depto. de Gen\u00e9tica, Universidade Federal do Paran\u00e1 Curitiba Paran\u00e1 81.531\u2010980 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Vicent","sequence":"additional","affiliation":[{"name":"Centro de Protecci\u00f3n Vegetal y Biotecnolog\u00eda, Instituto Valenciano de Investigaciones Agrarias (IVIA)  Moncada, Valencia 46113 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takehiko","family":"Shimada","sequence":"additional","affiliation":[{"name":"National Institute of Fruit Tree Science (NIFTS), National Agriculture and Bio\u2010oriented Research Organization (NARO)  Shizuoka, Shizuoka 424\u20100292 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9853-366X","authenticated-orcid":false,"given":"Leandro","family":"Pe\u00f1a","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia Vegetal, Fundo de Defesa da Citricultura (Fundecitrus) Araraquara S\u00e3o Paulo 14807\u2013040 Brazil"},{"name":"Instituto de Biolog\u00eda Molecular 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