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In tobacco, the\n                    <jats:italic>va<\/jats:italic>\n                    resistance gene commonly used to control\n                    <jats:italic>Potato virus Y<\/jats:italic>\n                    (PVY) corresponds to a large deletion affecting the\n                    <jats:italic>eIF4E\u20101<\/jats:italic>\n                    gene on chromosome 21. Here, we compared the resistance durability conferred by various types of mutations affecting\n                    <jats:italic>eIF4E\u20101<\/jats:italic>\n                    (deletions of various sizes, frameshift or nonsense mutations). The \u2018large deletion\u2019 genotypes displayed the broadest and most durable resistance, whereas frameshift and nonsense mutants displayed a less durable resistance, with rapid and frequent apparition of resistance\u2010breaking variants. In addition, genetic and transcriptomic analyses revealed that resistance durability is strongly impacted by a complex genetic locus on chromosome 14, which contains three other\n                    <jats:italic>eIF4E<\/jats:italic>\n                    genes. One of these,\n                    <jats:italic>eIF4E\u20103<\/jats:italic>\n                    , is rearranged as a hybrid gene between\n                    <jats:italic>eIF4E\u20102<\/jats:italic>\n                    and\n                    <jats:italic>eIF4E\u20103<\/jats:italic>\n                    (\n                    <jats:italic>\n                      eIF4E\u2010\n                      <jats:sup>2\u20103<\/jats:sup>\n                    <\/jats:italic>\n                    ) in the genotypes showing the most durable resistance, while\n                    <jats:italic>eIF4E\u20102<\/jats:italic>\n                    is differentially expressed between the tested varieties. RNA\u2010seq and quantitative reverse transcriptase\u2010polymerase chain reaction experiments demonstrated that\n                    <jats:italic>eIF4E\u20102<\/jats:italic>\n                    expression level is positively correlated with resistance durability. These results suggest that besides the nature of the mutation affecting\n                    <jats:italic>eIF4E\u20101<\/jats:italic>\n                    , three factors linked with a complex locus may potentially impact\n                    <jats:italic>va<\/jats:italic>\n                    durability: loss of an integral\n                    <jats:italic>eIF4E\u20103<\/jats:italic>\n                    , presence of\n                    <jats:italic>\n                      eIF4E\u2010\n                      <jats:sup>2\u20103<\/jats:sup>\n                    <\/jats:italic>\n                    and overexpression of\n                    <jats:italic>eIF4E\u20102.<\/jats:italic>\n                    This latter gene might act as a decoy in a non\u2010productive virus\u2013plant interaction, limiting the ability of PVY to evolve towards resistance breaking. Taken together, these results show that\n                    <jats:italic>va<\/jats:italic>\n                    resistance durability can in large part be explained by complex redundancy effects in the\n                    <jats:italic>eIF4E<\/jats:italic>\n                    gene family.\n                  <\/jats:p>","DOI":"10.1111\/mpp.12810","type":"journal-article","created":{"date-parts":[[2019,5,22]],"date-time":"2019-05-22T04:20:08Z","timestamp":1558498808000},"page":"1051-1066","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["A complex\n                    <i>eIF4E locus<\/i>\n                    impacts the durability of\n                    <i>va<\/i>\n                    resistance to\n                    <i>Potato virus Y<\/i>\n                    in tobacco"],"prefix":"10.1111","volume":"20","author":[{"given":"Vincent","family":"Michel","sequence":"first","affiliation":[{"name":"UMR 1332 Biologie du Fruit et Pathologie INRA, University Bordeaux 71 Av. E. Bourlaux Villenave d\u2019Ornon Cedex CS 20032 33882 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Julio","sequence":"additional","affiliation":[{"name":"Seita Imperial Tobacco La Tour 24100 Bergerac France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Candresse","sequence":"additional","affiliation":[{"name":"UMR 1332 Biologie du Fruit et Pathologie INRA, University Bordeaux 71 Av. E. Bourlaux Villenave d\u2019Ornon Cedex CS 20032 33882 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Cotucheau","sequence":"additional","affiliation":[{"name":"Seita Imperial Tobacco La Tour 24100 Bergerac France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Decorps","sequence":"additional","affiliation":[{"name":"Seita Imperial Tobacco La Tour 24100 Bergerac France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roxane","family":"Volpatti","sequence":"additional","affiliation":[{"name":"Seita Imperial Tobacco La Tour 24100 Bergerac France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3081-1034","authenticated-orcid":false,"given":"Beno\u00eet","family":"Moury","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathologie V\u00e9g\u00e9tale, INRA, Centre Recherche PACA, Domaine Saint Maurice Montfavet Cedex CS 60094 F84143 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Glais","sequence":"additional","affiliation":[{"name":"UMR IGEPP INRA, Domaine de la Motte BP 35327 Le Rheu Cedex 35653 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Jacquot","sequence":"additional","affiliation":[{"name":"INRA\u2010Cirad\u2010Supagro Montpellier, UMR BGPI Montpellier Cedex 34398 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois Dorlhac","family":"de Borne","sequence":"additional","affiliation":[{"name":"Seita Imperial Tobacco La Tour 24100 Bergerac France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Decroocq","sequence":"additional","affiliation":[{"name":"UMR 1332 Biologie du Fruit et Pathologie INRA, University Bordeaux 71 Av. 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